The quorum-sensing systems of Vibrio campbellii DS40M4 and BB120 are genetically and functionally distinct.
The quorum-sensing systems of Vibrio campbellii DS40M4 and BB120 are genetically and functionally distinct.
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cambelllii弧菌DS40M4和BB120的群体感应系统在遗传和功能上是不同的。
DOI：
10.1111/1462-2920.15602
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发表时间：
2021-09
影响因子：
5.1
通讯作者：
van Kessel JC
中科院分区：
文献类型：
--
作者：
Simpson CA;Petersen BD;Haas NW;Geyman LJ;Lee AH;Podicheti R;Pepin R;Brown LC;Rusch DB;Manzella MP;Papenfort K;van Kessel JC
Campbellii弧菌BB120(以前被归类为哈维弧菌)是研究弧菌群体感应的基本模式菌株。对Genbank中已测序的弧菌进行了系统发育分析，结果表明BB120与环境菌株V.campbellii DS40M4亲缘关系密切。我们利用DS40M4的S吸收外源DNA的能力，快速产生了编码BB120群体感应系统同源基因缺失的>；30株等基因菌株。我们的结果表明，DS40M4的群体感应电路与BB120的群体感应电路有三个不同之处：1)DS40M4不产生酰基高丝氨酸内酯自身诱导剂，而是编码一个活性的孤儿LuxN受体；2)群体调节小RNA(QRR)不是由自身诱导信号通过响应调节器Luxo单独调节的；3)DS40M4的群体感应调节子比BB120小得多(分别为~100个基因和~400个基因)。利用比较基因组学来扩展我们对群体感应电路多样性的理解，我们观察到LuxM/LuxN蛋白的保守性在弧菌物种之间和内部都有很大的不同。这些菌株也是表型不同的：DS40M4表现出更强的细菌间细胞杀伤力，而BB120形成更强大的生物膜，并是生物发光的。这些结果强调了需要检查野生分离物，以更广泛地了解海洋生态系统中的细菌多样性。
Vibrio campbellii BB120 (previously classified as Vibrio harveyi) is a fundamental model strain for studying quorum sensing in vibrios. A phylogenetic evaluation of sequenced Vibrio strains in Genbank revealed that BB120 is closely related to the environmental isolate V. campbellii DS40M4. We exploited DS40M4’s competence for exogenous DNA uptake to rapidly generate >30 isogenic strains with deletions of genes encoding BB120 quorum-sensing system homologs. Our results show that the quorum-sensing circuit of DS40M4 is distinct from BB120 in three ways: 1) DS40M4 does not produce an acyl homoserine lactone autoinducer but encodes an active orphan LuxN receptor, 2) the quorum regulatory small RNAs (Qrrs) are not solely regulated by autoinducer signaling through the response regulator LuxO, and 3) the DS40M4 quorum-sensing regulon is much smaller than BB120 (~100 genes vs ~400 genes, respectively). Using comparative genomics to expand our understanding of quorum-sensing circuit diversity, we observe that conservation of LuxM/LuxN proteins differs widely both between and within Vibrio species. These strains are also phenotypically distinct: DS40M4 exhibits stronger interbacterial cell killing, whereas BB120 forms more robust biofilms and is bioluminescent. These results underscore the need to examine wild isolates for a broader view of bacterial diversity in the marine ecosystem.
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影响因子：
8.4
作者：
Bagert JD;van Kessel JC;Sweredoski MJ;Feng L;Hess S;Bassler BL;Tirrell DA
通讯作者：
Tirrell DA
DOI：
10.1007/978-1-4939-8685-9_6
发表时间：
2018-01-01
期刊：
VIBRIO CHOLERAE: METHODS AND PROTOCOLS
影响因子：
--
作者：
Dalia, Ankur B.
通讯作者：
Dalia, Ankur B.
影响因子：
3.6
作者：
Freeman, JA;Bassler, BL
通讯作者：
Bassler, BL
sigma(E) 介导的温度计控制溶藻弧菌从 LuxR 介导的毒力基因表达到热应激适应的转变
影响因子：
6.7
作者：
Gu D;Guo M;Yang M;Zhang Y;Zhou X;Wang Q
通讯作者：
Wang Q
影响因子：
3.2
作者：
Henke, JM;Bassler, BL
通讯作者：
Bassler, BL