High Vimentin Expression Associated with Lymph Node Metastasis and Predicated a Poor Prognosis in Oral Squamous Cell Carcinoma.
High Vimentin Expression Associated with Lymph Node Metastasis and Predicated a Poor Prognosis in Oral Squamous Cell Carcinoma.
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波形蛋白高表达与淋巴结转移相关，预示口腔鳞状细胞癌预后不良。
DOI：
10.1038/srep38834
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发表时间：
2016-12-14
影响因子：
4.6
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Liu S;Liu L;Ye W;Ye D;Wang T;Guo W;Liao Y;Xu D;Song H;Zhang L;Zhu H;Deng J;Zhang Z
口腔鳞状细胞癌（OSCC）是全球常见的公共卫生问题，预后不良，这在很大程度上是由于淋巴结转移和复发。鉴定具有淋巴结转移的口腔鳞状细胞癌的特异性分子标志物对于早期特异性诊断非常重要。在本研究中，我们通过无偏转录组微阵列分析在一对口腔鳞状细胞癌细胞系（具有淋巴结转移的HN12细胞系和低转移的亲本HN4细胞系）中筛选潜在的预后标志物。结果显示，与非转移性HN4细胞相比，波形蛋白在转移性HN12细胞中表达增加了87倍，在所有上调基因中位居首位。用转化生长因子-β1处理非转移性HN4细胞可诱导上皮 - 间质转化（EMT），同时波形蛋白表达增加以及迁移活性增强。同样，通过小干扰RNA敲低波形蛋白可抑制HN12细胞的侵袭和迁移活性，这表明波形蛋白在口腔鳞状细胞癌细胞的上皮 - 间质转化相关功能中起重要作用。最后，免疫组织化学（IHC）染色分析表明，波形蛋白高表达与口腔鳞状细胞癌患者的高淋巴结转移（p < 0.05）和总体生存率差（p < 0.05）密切相关。因此，波形蛋白高表达与转移潜能增加密切相关，并可作为口腔鳞状细胞癌患者预后不良的预测标志物。
Oral squamous cell carcinoma (OSCC) is a common public health problem worldwide with poor prognosis, which is largely due to lymph node metastasis and recurrence. Identification of specific molecular markers of OSCC with lymph node metastasis would be very important for early and specific diagnosis. In this study, we screened for the potential prognosis markers via unbiased transcriptomic microarray analysis in paired two OSCC cell lines, a lymph node metastatic HN12 cell line and a low metastatic parental HN4 cell line. The results showed that vimentin, with 87-fold increase of expression, was on the top of all upregulated genes in metastatic HN12 cells compared to non-metastatic HN4 cells. Treatment of non-metastatic HN4 cells with TGF-β1 induced epithelial to mesenchymal transition (EMT), with increased vimentin expression as well as enhanced migration activity. Consistently, knockdown of vimentin via siRNA resulted in suppressed invasion and migration activities of HN12 cells, suggesting an essential role of vimentin in EMT-related functions of OSCC cells. Finally, immunohistochemical (IHC) staining analysis showed that high vimentin expression was strongly associated with high lymph node metastases (p < 0.05), and poor overall survival (p < 0.05) in OSCC patients. Thus, high vimentin expression is strongly associated with increased metastatic potential, and may serve as a prediction marker for poor prognosis in OSCC patients.
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自分泌上皮调节蛋白通过 EMT 激活唾液腺样囊性癌肺转移的 EGFR 通路
影响因子：
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作者：
Liu S;Ye D;Xu D;Liao Y;Zhang L;Liu L;Yu W;Wang Y;He Y;Hu J;Guo W;Wang T;Sun B;Song H;Yin H;Liu J;Wu Y;Zhu H;Zhou BP;Deng J;Zhang Z
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DOI：
10.1073/pnas.80.9.2618
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
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作者：
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4
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通讯作者：
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作者：
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DOI：
10.1002/hed.2890080202
发表时间：
1985-01-01
期刊：
HEAD & NECK SURGERY
影响因子：
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作者：
GRANDI, C;ALLOISIO, M;MOLINARI, R
通讯作者：
MOLINARI, R