P2Y receptor‐mediated Ca2+ signalling in cultured rat aortic smooth muscle cells
P2Y receptor‐mediated Ca2+ signalling in cultured rat aortic smooth muscle cells
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培养的大鼠主动脉平滑肌细胞中 P2Y 受体介导的 Ca2+ 信号传导
DOI：
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发表时间：
1999
影响因子：
7.3
通讯作者：
S. Wilson
中科院分区：
文献类型：
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作者：
J. Pediani;J. McGrath;S. Wilson
ATP、UTP、ADP和ADP-β-S在培养的主动脉平滑肌细胞中引起Ca 2 +-信号，尽管ADP、UDP和ADP-β-S的最大反应是ATP和UTP的约40%。腺苷、AMP或α，β-亚甲基-ATP无影响。这些反应归因于P2 Y2/4和P2 Y1受体，我们假设它们可以分别被UTP和ADP-β-S选择性激活。百日咳毒素降低了对UTP的反应（约50%），而该毒素对ADP-β-S的反应没有影响。这表明P2 Y2/4受体同时与百日咳毒素敏感性和耐药性G蛋白偶联，而P2 Y1受体仅与毒素耐药性蛋白偶联。用UTP或ADP-β-S重复刺激引起脱敏，12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）可增强脱敏，星形孢菌素可减弱脱敏。TPA完全消除了对ADP-β-S的敏感性，但对UTP的反应具有TPA抵抗成分。然而，在百日咳毒素处理的细胞中，TPA可以完全消除对UTP的敏感性，因此这种反应的TPA抗性部分似乎是由百日咳毒素敏感的G蛋白介导的。当用这种核苷酸反复刺激百日咳毒素处理的细胞时，不会发生对UTP的敏感性丧失，这表明百日咳毒素敏感的G蛋白介导了这种效应。然而，毒素并不影响对ADP-β-S的脱敏。
ATP, UTP, ADP and ADP‐β‐S elicited Ca2+‐signals in cultured aortic smooth muscle cells although ADP, UDP and ADP‐β‐S gave ∼40% of the maximal response seen with ATP and UTP. Adenosine, AMP or α,β‐methylene‐ATP had no effect. These responses were attributed to P2Y2/4 and P2Y1 receptors, which we assumed could be selectively activated by UTP and ADP‐β‐S respectively. The response to UTP was reduced (∼50%) by pertussis toxin, whilst this toxin had no effect upon the response to ADP‐β‐S. This suggests P2Y2/4 receptors simultaneously couple to pertussis toxin‐sensitive and ‐resistant G proteins whilst P2Y1 receptors couple to only the toxin‐resistant proteins. Repeated stimulation with UTP or ADP‐β‐S caused desensitization which was potentiated by 12‐O‐tetradecanoyl phorbol‐13‐acetate (TPA) and attenuated by staurosporine. TPA completely abolished sensitivity to ADP‐β‐S but the response to UTP had a TPA‐resistant component. In pertussis toxin‐treated cells, however, TPA could completely abolish sensitivity to UTP and so the TPA‐resistant part of this response seems to be mediated by pertussis toxin‐sensitive G proteins. Loss of sensitivity to UTP did not occur when pertussis toxin‐treated cells were repeatedly stimulated with this nucleotide, suggesting that pertussis toxin‐sensitive G proteins mediate this effect. The toxin did not, however affect desensitization to ADP‐β‐S.
三种磷脂酶 C 激活 P2 受体的尿苷核苷酸选择性：鉴定 UDP 选择性、UTP 选择性以及 ATP 和 UTP 特异性受体。
DOI：
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发表时间：
1996
期刊：
Molecular pharmacology.
影响因子：
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作者：
Nicholas,RA;Watt,WC;Lazarowski,ER;Li,Q;Harden,K
通讯作者：
Harden,K
影响因子：
13.8
作者：
Fredholm, BB;Abbracchio, MP;Williams, M
通讯作者：
Williams, M
肺泡 II 型细胞 P2u 嘌呤能受体的克隆和表达。
DOI：
10.1165/ajrcmb.12.1.7811468
发表时间：
1995
期刊：
American journal of respiratory cell and molecular biology.
影响因子：
--
作者：
Rice,WR;Burton,FM;Fiedeldey,DT
通讯作者：
Fiedeldey,DT