A critical role of Sp1 transcription factor in regulating the human Ki-67 gene expression
A critical role of Sp1 transcription factor in regulating the human Ki-67 gene expression
复制标题
Sp1转录因子在调节人类Ki-67基因表达中的关键作用
DOI：
10.1007/s13277-010-0119-4
复制
发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
郑骏年
中科院分区：
文献类型：
--
作者：
郑骏年
Ki-67 plays a crucial role in cell proliferation as well as maintenance or regulation of cell division. The mechanism governing the Ki-67 gene expression remains unknown. Thus, we cloned the core promoter of the human Ki-67 gene and further investigated its transcriptional regulation. The putative Sp1 binding sites were confirmed by electrophoretic mobility shift assay together with an anti-Sp1 antibody-mediated supershift assay. Deletion mutagenesis and firefly luciferase reporter gene assay demonstrated the essential contribution of Sp1 on transcriptional activation of the Ki-67 gene. In this study, we first confirm that there are three Sp1 binding sites in the Ki-67 core promoter. Two Sp1 sites (one at position −159 to −145 nt and the other at position −14 to +12 nt) are mainly involved in transcriptional regulation of the Ki-67 gene. Overexpression of Sp1 can enhance the Ki-67 promoter activity. However, down-regulation of Sp1 expression using siRNA-Sp1 and mithramycin effectively inhibits the Ki-67 gene transcription. Our results suggest that Sp1 is essential for basal promoter activity of the human Ki-67 gene. Inhibition of the Ki-67 transcriptional activity through abolishment of Sp1 may provide the useful prospect for gene therapy.
登录
查看更多内容
影响因子：
5.2
作者：
Y. Hosoi;Toshiaki Watanabe;K. Nakagawa;Y. Matsumoto;A. Enomoto;A. Morita;H. Nagawa;N. Suzuki
通讯作者：
Y. Hosoi;Toshiaki Watanabe;K. Nakagawa;Y. Matsumoto;A. Enomoto;A. Morita;H. Nagawa;N. Suzuki
影响因子：
11.2
作者：
Q. Shi;X. Le;J. Abbruzzese;Zhihai Peng;C. Qian;Hong-wei Tang;Q. Xiong;Bailiang Wang;Xiang Chen Li-X
通讯作者：
Q. Shi;X. Le;J. Abbruzzese;Zhihai Peng;C. Qian;Hong-wei Tang;Q. Xiong;Bailiang Wang;Xiang Chen Li-X
影响因子：
4.8
作者：
Jang, SI;Steinert, PM
通讯作者：
Steinert, PM
影响因子：
4.5
作者：
Kausch, I;Jiang, H;Jocham, D
通讯作者：
Jocham, D
影响因子：
2.9
作者：
Li, L;He, SH;Davie, JR
通讯作者：
Davie, JR