Genome-Wide Analysis and Functional Characterization of Pyruvate Kinase (PK) Gene Family Modulating Rice Yield and Quality.
Genome-Wide Analysis and Functional Characterization of Pyruvate Kinase (PK) Gene Family Modulating Rice Yield and Quality.
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DOI：
10.3390/ijms232315357
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发表时间：
2022-12-05
影响因子：
5.6
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中科院分区：
文献类型：
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丙酮酸激酶（PK）是糖酵解的三大限速酶之一，在能量代谢中起着关键作用。在这项研究中，我们已经确定了10个PK基因从水稻基因组。最初，这些基因被分为两类：胞质丙酮酸激酶（PKC）和质体丙酮酸激酶（PKp）。然后，表达分析显示OsPK 1、OsPK 3、OsPK 4、OsPK 6和OsPK 9在谷粒中高度表达。此外，PK可以形成杂聚物。此外，发现阿坝显著调节水稻PK基因（OsPK 1、OsPK 4、OsPK 9和OsPK 10）的表达。有趣的是，所有的基因都被发现在很大程度上参与了稻米品质和产量的调控。例如，OsPK 3、OsPK 5、OsPK 7、OsPK 8和OsPK 10以及OsPK 4、OsPK 5、OsPK 6和OsPK 10的破坏分别降低了千粒重和结实率。此外，与WT相比，通过CRISPR/Cas9系统破坏OsPK 4、OsPK 6、OsPK 8和OsPKlO显示总淀粉含量增加和蛋白质含量降低。类似地，OsPK 4、OsPK 8和OsPK 10基因的操作增加了直链淀粉含量。同时，与野生型相比，除了ospk 6之外，所有CRISPR突变体和RNAi品系的谷粒显示出垩白率的显著增加。总体而言，本研究的PK基因家族的所有基因的功能，并显示其尚未开发的潜力，以提高水稻产量和品质性状。
Pyruvate kinase (PK) is one of the three rate-limiting enzymes of glycolysis, and it plays a pivotal role in energy metabolism. In this study, we have identified 10 PK genes from the rice genome. Initially, these genes were divided into two categories: cytoplasmic pyruvate kinase (PKc) and plastid pyruvate kinase (PKp). Then, an expression analysis revealed that OsPK1, OsPK3, OsPK4, OsPK6, and OsPK9 were highly expressed in grains. Moreover, PKs can form heteropolymers. In addition, it was found that ABA significantly regulates the expression of PK genes (OsPK1, OsPK4, OsPK9, and OsPK10) in rice. Intriguingly, all the genes were found to be substantially involved in the regulation of rice grain quality and yield. For example, the disruption of OsPK3, OsPK5, OsPK7, OsPK8, and OsPK10 and OsPK4, OsPK5, OsPK6, and OsPK10 decreased the 1000-grain weight and the seed setting rate, respectively. Further, the disruption of OsPK4, OsPK6, OsPK8, and OsPK10 through the CRISPR/Cas9 system showed an increase in the content of total starch and a decrease in protein content compared to the WT. Similarly, manipulations of the OsPK4, OsPK8, and OsPK10 genes increased the amylose content. Meanwhile, the grains of all CRISPR mutants and RNAi lines, except ospk6, showed a significant increase in the chalkiness rate compared to the wild type. Overall, this study characterizes the functions of all the genes of the PK gene family and shows their untapped potential to improve rice yield and quality traits.
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