Molecular diversity of HLA‐A, ‐B and ‐C alleles in a North Indian population as determined by PCR‐SSOP
Molecular diversity of HLA‐A, ‐B and ‐C alleles in a North Indian population as determined by PCR‐SSOP
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通过 PCR-SSOP 测定北印度人群 HLA-A、-B 和 -C 等位基因的分子多样性
DOI：
10.1111/j.1744-313x.2007.00677.x
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发表时间：
2007
影响因子：
2.2
通讯作者：
P. Stastny
中科院分区：
文献类型：
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作者：
R. Rani;C. Marcos;A. Lazaro;Y. Zhang;P. Stastny
我们使用分子方法，通过聚合酶链反应和序列特异性寡核苷酸探针杂交，确定了印度北部正常、健康、无关个体中人类白细胞抗原（HLA）-A、-B和-C等位基因的频率，因为没有综合报告显示印度北部存在的所有I类等位基因的分子多样性。A基因座的等位基因A*0101、A*0206、A*0301、A*1101、A*6801、A*2401和A*3101占总样本数的91.11%。在HLA-B基因座上，共观察到47个B基因座等位基因，唯一发现的等位基因频率为15%的等位基因是B*5801。观察到的其他常见B基因座等位基因为B*5101、B*3503和B*4006，相对较不常见的等位基因为B*5201、B*3501、B*0702、B*4403、B*5701、B*1801和B*5501。在所研究的样本中，92.31%的样本为B-位点等位基因杂合子。Cw*0602和Cw*0401是最常见的C位点等位基因。其他常见的C基因座等位基因为Cw*0102、Cw*0302、Cw * 0701、Cw*0702、Cw*1202、Cw *1203、Cw*1502和Cw*1503。除了东方等位基因B*1301、B*1502和B*4001外，在北印度人中还观察到非洲人常见的HLA等位基因B*5801、A*68012、B*5301、B*44032、B*4006和Cw*1701，这证实北印度人的遗传组成是具有蒙古人种和黑人人种元素的高加索人种。也观察到一些新的/罕见的等位基因，如B*1802，描述为来自泰国的新等位基因和B*8101，早期在Bubi人群中描述，尽管频率较低，显示了北印度人中存在的HLA I类等位基因的多样性。
We have used molecular methods to determine the frequencies of human leukocyte antigen (HLA)‐A, ‐B and ‐C alleles in normal, healthy, unrelated individuals from North India using polymerase chain reaction and hybridization with sequence‐specific oligonucleotide probes as there is no comprehensive report showing molecular diversity of all the class‐I alleles present in North Indians. A*0101, A*0206, A*0301, A*1101, A*6801, A*2401 and A*3101 were the most prevalent alleles of the A locus with 91.11% of the samples showing heterozygosity. At the HLA‐B locus a total of 47 B locus alleles were observed and the only allele found with an allele frequency of 15% was B*5801. Other frequent B‐locus alleles observed were B*5101, B*3503 and B*4006 with relatively less frequent alleles like B*5201, B*3501, B*0702, B*4403, B*5701, B*1801 and B*5501. Of the samples studied 92.31% were heterozygous for B‐locus alleles. Cw*0602 and Cw*0401 were the most frequent C‐locus alleles. Other frequent C‐locus alleles were Cw*0102, Cw*0302, Cw*0701, Cw*0702, Cw*1202, Cw*1203, Cw*1502 and Cw*1503. HLA alleles common in Africans like B*5801, A*68012, B*5301, B*44032, B*4006 and Cw*1701 were observed in the North Indians besides oriental alleles like B*1301, B*1502 and B*4001 confirming that the genetic make‐up of North Indians is Caucasoid with elements of Mongoloid and Negrito races. Some new/rare alleles like B*1802, described as a new allele from Thailand and B*8101, described earlier in a Bubi population were also observed although with low frequencies, showing the diversity of HLA class‐I alleles present in the North Indians.
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影响因子：
158.5
作者：
Gao, XJ;Nelson, GW;Carrington, M
通讯作者：
Carrington, M
印度北部人群中的 HLA-DPB1 等位基因和新变体的描述 (DPB1*5601)。
影响因子：
--
作者：
Rani,R;Fernandez-Viña,MA;Zhang,S;Stastny,P
通讯作者：
Stastny,P
HLA-B17 抗原组的等位基因频率：通过血清学、IEF 和 PCR-SSOP 分型进行比较分析。
影响因子：
--
作者：
Levine,JE;Yang,SY
通讯作者：
Yang,SY
影响因子：
56.9
作者：
Carrington, M;Nelson, GW;O'Brien, SJ
通讯作者：
O'Brien, SJ