The molecular pathogenesis of achalasia: a paired lower esophageal sphincter muscle and serum 4D label-free proteomic study.
The molecular pathogenesis of achalasia: a paired lower esophageal sphincter muscle and serum 4D label-free proteomic study.
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DOI：
10.1093/gastro/goad031
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发表时间：
2023
影响因子：
3.6
通讯作者：
Xiao, Yinglian
中科院分区：
文献类型：
--
作者：
Chen, Songfeng;Xing, Xiangbin;Hou, Xun;Zhuang, Qianjun;Tan, Niandi;Cui, Yi;Wang, Jinhui;Zhang, Mengyu;Hu, Shixian;Xiao, Yinglian
Achalasia is a primary esophageal motility disorder with potential molecular pathogenesis remaining uncertain. This study aimed to identify the differentially expressed proteins and potential pathways among achalasia subtypes and controls to further reveal the molecular pathogenesis of achalasia. Paired lower esophageal sphincter (LES) muscle and serum samples from 24 achalasia patients were collected. We also collected 10 normal serum samples from healthy controls and 10 normal LES muscle samples from esophageal cancer patients. The 4D label-free proteomic analysis was performed to identify the potential proteins and pathways involved in achalasia. Analysis of Similarities showed distinct proteomic patterns of serum and muscle samples between achalasia patients and controls (both P < 0.05). Functional enrichment analysis suggested that these differentially expressed proteins were immunity-, infection-, inflammation-, and neurodegeneration-associated. The mfuzz analysis in LES specimens showed that proteins involved in the extracellular matrix–receptor interaction increased sequentially between the control group, type III, type II, and type I achalasia. Only 26 proteins altered in the same directions in serum and muscle samples. This first 4D label-free proteomic study of achalasia indicated that there were specific protein alterations in both the serum and muscle of achalasia, involving immunity, inflammation, infection, and neurodegeneration pathways. Distinct protein clusters between types I, II, and III revealed the potential molecular pathways associated with different disease stages. Analysis of proteins changed in both muscle and serum samples highlighted the importance of further studies on LES muscle and revealed potential autoantibodies.
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影响因子：
3.4
作者：
Im SK;Yeo M;Lee KJ
通讯作者：
Lee KJ
影响因子：
3.5
作者：
Priego-Ranero, Angel;Opdenakker, Ghislain;Torres-Villalobos, Gonzalo
通讯作者：
Torres-Villalobos, Gonzalo
影响因子：
9.8
作者：
Facco, Monica;Brun, Paola;Zaninotto, Giovanni
通讯作者：
Zaninotto, Giovanni
ACG临床指南：aChalasia的诊断和管理。
DOI：
10.14309/ajg.0000000000000731
发表时间：
2020-09
期刊：
The American journal of gastroenterology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.6
作者：
Lata, Tahmina;Trautman, Jodie;Townend, Philip;Wilson, Robert B.
通讯作者：
Wilson, Robert B.