Deinococcus arenae sp. nov., a novel species isolated from sand in South Korea
Deinococcus arenae sp. nov., a novel species isolated from sand in South Korea
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奇异球菌 sp.
DOI：
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
T. Seo
中科院分区：
文献类型：
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作者：
Dongwook Lee;S. Cha;J. Jang;T. Seo
从韩国阳阳海滩的沙标本中分离出一株命名为SA1T的革兰氏阴性菌株。观察到SA1T细胞为红色色素，严格有氧，无运动棒。菌株SA1T在30°C和pH 7 （pH生长范围为pH 7 - 9）条件下生长最佳。对SA1T的16S rRNA基因序列的系统发育分析表明，该生物属于热球菌门，与放射线球菌BM2T是Deinococcus- thermus属的一个分支，是Deinococcus actinosclerus BM2T的近亲（序列相似性为99.8%）。该菌株过氧化氢酶呈阳性，氧化酶呈阴性，代谢2-酮葡萄糖酸盐、醋酸盐、d- 3-羟基丁酸盐、d-葡萄糖、d-麦芽糖、d-甘露醇、d-甘露糖、d-蜜二糖、d-山梨醇、d-蔗糖、糖原、l-丙氨酸、l-组氨酸、l-苹果酸盐、l-脯氨酸和n-戊酸盐。通过热变性法测定DNA的鸟嘌呤加胞嘧啶（G + C）碱基组成为69.5 mol %。主要的呼吸醌被鉴定为含有8个异戊二烯单位的甲基萘醌（MK-8）。菌株SA1T的极性脂质谱显示存在几种未知的糖脂、磷酸糖脂、磷脂、氨基磷脂和未知的脂质。此外，菌株SA1T最丰富的脂肪酸为C15:1ω6c、C16:1ω7c和C17:0。基于系统发育分析、DNA-DNA杂交和生物化学特征，认为菌株SA1T （=KCTC 33741T = JCM 31047T）是Deinococcus属的一个新种，建议将其命名为Deinococcus arenae sp. 11。
A Gram-negative strain, designated SA1T, was isolated from a sand specimen from Yangyang Beach, South Korea. The cells of SA1T were observed to be red pigmented, strictly aerobic, non-motile rods. Strain SA1T was found to grow optimally at 30 °C and pH 7 (pH growth range, pH 7–9). Phylogenetic analyses of the 16S rRNA gene sequence of SA1T showed that the organism belongs to the phylum Deinococcus-Thermus and forms a branch within the genus Deinococcus, with Deinococcus actinosclerus BM2T as a close relative (99.8 % sequence similarity). The strain was found to be catalase positive and oxidase negative and to metabolise 2-ketogluconate, acetate, d,l-3-hydroxybutyrate, d-glucose, d-maltose, d-mannitol, d-mannose, d-melibiose, d-sorbitol, d-sucrose, glycogen, l-alanine, l-histidine, l-malate, l-proline and n-valerate. The guanine plus cytosine (G + C) base composition of the DNA was 69.5 mol %, as determined by the thermal denaturation method. The predominant respiratory quinone was identified as menaquinone with eight isoprene units (MK-8). The polar lipid profile of strain SA1T showed the presence of several unidentified glycolipids, phosphoglycolipids, phospholipids, aminophospholipids and unidentified lipids. In addition, the most abundant fatty acids of strain SA1T were identified as C15:1ω6c, C16:1ω7c and C17:0. On the basis of phylogenetic analyses, DNA–DNA hybridisation and biochemical characteristics, strain SA1T (=KCTC 33741T = JCM 31047T) is concluded to represent a novel species of the genus Deinococcus, for which the name Deinococcus arenae sp. nov. is proposed.
影响因子：
56.9
作者：
White, O;Eisen, JA;Fraser, CM
通讯作者：
Fraser, CM