EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories.
EUCAST rapid antimicrobial susceptibility testing (RAST) in blood cultures: validation in 55 European laboratories.
复制标题
DOI：
10.1093/jac/dkaa333
复制
发表时间：
2020-11-01
期刊：
影响因子：
--
通讯作者：
RAST Study Group
中科院分区：
文献类型：
--
作者：
Åkerlund A;Jonasson E;Matuschek E;Serrander L;Sundqvist M;Kahlmeter G;RAST Study Group
当血液感染是由耐药细菌引起时，快速药敏试验(RAST)对调整治疗非常重要。EUCAST RAST方法直接来自阳性血液培养，在欧洲的一项多实验室研究中得到了验证。北欧(NE)的40个实验室和南欧(SE)的15个实验室从临床血培养中检出大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、金黄色葡萄球菌或肺炎链球菌。将孵育4、6和8 h的分类结果与EUCAST标准16-20 h纸片扩散的结果进行比较。对方法、初步断点和实验室的性能进行了评估。NE/SE分离株总数为833/318株。随着孵育时间的延长(4、6和8 h)，可读数(88%、96%和99%)和可解译数(70%、81%和85%)增加。分类一致性较好，NE/SE在4 h、6 h和8 h的总错误率分别为2.4%/4.9%、1.1%/3.5%和1.1%/3.3%，4、6和8 h的假敏感性分别低于0.3%和1.1%，相应的假抗性百分比低于1.9%和2.8%。在微调断点后，可以解释更多的区域(73%、89%和93%)，错误率仅受轻微影响。EUCAST RAST方法无需重大投资即可在常规实验室中实施。培养4-6 h后，可为相关血流感染病原菌提供可靠的药敏结果。
When bloodstream infections are caused by resistant bacteria, rapid antimicrobial susceptibility testing (RAST) is important for adjustment of therapy. The EUCAST RAST method, directly from positive blood cultures, was validated in a multi-laboratory study in Europe. RAST was performed in 40 laboratories in northern Europe (NE) and 15 in southern Europe (SE) from clinical blood cultures positive for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus or Streptococcus pneumoniae. Categorical results at 4, 6 and 8 h of incubation were compared with results for EUCAST standard 16–20 h disc diffusion. The method, preliminary breakpoints and the performance of the laboratories were evaluated. The total number of isolates was 833/318 in NE/SE. The number of zone diameters that could be read (88%, 96% and 99%) and interpreted (70%, 81% and 85%) increased with incubation time (4, 6 and 8 h). The categorical agreement was acceptable, with total error rates in NE/SE of 2.4%/4.9% at 4 h, 1.1%/3.5% at 6 h and 1.1%/3.3% at 8 h. False susceptibility at 4, 6 and 8 h of incubation was below 0.3% and 1.1% in NE and SE, respectively, and the corresponding percentages for false resistance were below 1.9% and 2.8%. After fine-tuning breakpoints, more zones could be interpreted (73%, 89% and 93%), with only marginally affected error rates. The EUCAST RAST method can be implemented in routine laboratories without major investments. It provides reliable antimicrobial susceptibility testing results for relevant bloodstream infection pathogens after 4–6 h of incubation.
登录
查看更多内容
影响因子：
2.7
作者：
Li Y;Yang X;Zhao W
通讯作者：
Zhao W
影响因子：
19
作者：
Brown, D.;Canton, R.;Kahlmeter, G.
通讯作者：
Kahlmeter, G.
影响因子：
5.2
作者：
Jonasson, Emma;Matuschek, Erika;Kahlmeter, Gunnar
通讯作者：
Kahlmeter, Gunnar
影响因子：
9.4
作者：
COYLE, MB;MCGONAGLE, LA;SCHOENKNECHT, FD
通讯作者：
SCHOENKNECHT, FD
DOI：
10.1007/s10096-020-03846-3
发表时间：
2020-02-28
影响因子：
4.5
作者：
Jasuja, Jasmin Kaur;Zimmermann, Stefan;Burckhardt, Irene
通讯作者：
Burckhardt, Irene