Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.
Emerging Microtechnologies and Automated Systems for Rapid Bacterial Identification and Antibiotic Susceptibility Testing.
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DOI：
10.1177/2472630317727519
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发表时间：
2017-12
期刊：
影响因子：
2.7
通讯作者：
Zhao W
中科院分区：
文献类型：
--
作者：
Li Y;Yang X;Zhao W
由于耐药细菌的增加，快速细菌鉴定（ID）和抗生素敏感性测试（AST）的需求量很大。传统的基于培养的AST方法存在周转时间长的问题。出于必要，医生通常不得不凭经验使用抗生素治疗患者，这导致了抗生素的不适当使用，死亡率和医疗成本升高以及抗生素耐药性。微型化和自动化的最新进展为快速细菌ID/AST分析提供了有前途的解决方案，这将在未来几年内对感染性疾病的临床管理和抗生素管理产生重大影响。在这篇综述中，我们总结和分析了代表性的新兴微米和纳米技术，以及细菌ID/AST的自动化系统，包括表型（例如，基于微流体的细菌培养，以及单细胞的数字成像）和分子（例如，多重PCR、杂交探针、纳米颗粒、合成生物学工具、质谱和测序技术）方法。我们还讨论了具有代表性的护理点（POC）系统，该系统集成了样品处理，流体处理和快速细菌ID/AST检测。最后，我们强调了主要的剩余挑战，并讨论了未来可能的努力，以改善快速细菌ID/AST技术的临床结果。
Rapid bacterial identification (ID) and antibiotic susceptibility testing (AST) are in great demand due to the rise of drug-resistant bacteria. Conventional culture-based AST methods suffer from a long turnaround time. By necessity, physicians often have to treat patients empirically with antibiotics, which has led to an inappropriate use of antibiotics, an elevated mortality rate and healthcare costs, and antibiotic resistance. Recent advances in miniaturization and automation provide promising solutions for rapid bacterial ID/AST profiling, which will potentially make a significant impact in the clinical management of infectious diseases and antibiotic stewardship in the coming years. In this review, we summarize and analyze representative emerging micro- and nanotechnologies, as well as automated systems for bacterial ID/AST, including both phenotypic (e.g., microfluidic-based bacterial culture, and digital imaging of single cells) and molecular (e.g., multiplex PCR, hybridization probes, nanoparticles, synthetic biology tools, mass spectrometry, and sequencing technologies) methods. We also discuss representative point-of-care (POC) systems that integrate sample processing, fluid handling, and detection for rapid bacterial ID/AST. Finally, we highlight major remaining challenges and discuss potential future endeavors toward improving clinical outcomes with rapid bacterial ID/AST technologies.
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影响因子：
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DOI：
10.1073/pnas.1119540109
发表时间：
2012-04-17
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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