A Toll-Spätzle pathway in the tobacco hornworm, Manduca sexta.
A Toll-Spätzle pathway in the tobacco hornworm, Manduca sexta.
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Manduca Sexta烟草中的Toll-Spätzle通道。
DOI：
10.1016/j.ibmb.2012.03.009
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发表时间：
2012-07
影响因子：
3.8
通讯作者：
Yu, Xiao-Qiang
中科院分区：
文献类型：
--
作者：
Zhong, Xue;Xu, Xiao-Xia;Yi, Hui-Yu;Lin, Christopher;Yu, Xiao-Qiang
昆虫体内合成一系列抗菌肽，其表达受果蝇Toll和Imd（免疫缺陷）途径的调控。果蝇Toll通路是在Spätzle（Spz）被Spätzle加工酶（SPE）切割后被激活，释放出活性的C末端C106结构域（DmSpz-C106），然后与Toll受体结合，启动信号通路并调节AMP基因（如drosomycin）的表达。Toll和Spz基因已在其他昆虫中鉴定，但Toll和Spz之间的相互作用以及其他昆虫物种中Toll-Spz途径的直接证据尚未得到证实。我们的目的是研究烟草天蛾Toll-Spz通路，并比较M。sexta和D.黑胃Toll-Spz通路。免疫共沉淀（Co-IP）试验表明MsTollecto（M. Sexta Toll）能与MsSpz-C108（M. DmTollecto能与DmSpz-C106相互作用，但不能与DmSpz相互作用，提示Toll受体仅与Spz的活性C端结构域结合。MsToll-MsSpz-C108复合物在果蝇S2细胞中的共表达可上调Drosomycin基因的表达，表明MsToll-MsSpz-C108复合物可激活Toll信号通路。体内实验表明，在M.预先注射MsToll抗体可阻断MsSpz-C108对六龄幼虫的诱导作用，进一步证实了M. toll-Spz途径的存在。sexta，一种鳞翅目昆虫。
Insects synthesize a battery of antimicrobial peptides (AMPs) and expression of AMP genes is regulated by the Toll and Imd (immune deficiency) pathways in Drosophila melanogaster. Drosophila Toll pathway is activated after Spätzle (Spz) is cleaved by Spätzle processing enzyme (SPE) to release the active C-terminal C106 domain (DmSpz-C106), which then binds to the Toll receptor to initiate the signaling pathway and regulate expression of AMP genes such as drosomycin. Toll and Spz genes have been identified in other insects, but interaction between Toll and Spz and direct evidence for a Toll-Spz pathway in other insect species have not been demonstrated. Our aim is to investigate a Toll-Spz pathway in Manduca sexta, and compare M. sexta and D. melanogaster Toll-Spz pathways. Co-immunoprecipitation (Co-IP) assays showed that MsTollecto (the ecto-domain of M. sexta Toll) could interact with MsSpz-C108 (the active C-terminal C108 domain of M. sexta Spz) but not with full-length MsSpz, and DmTollecto could interact with DmSpz-C106 but not DmSpz, suggesting that Toll receptor only binds to the active C-terminal domain of Spz. Co-expression of MsToll-MsSpz-C108, but not MsToll-MsSpz, could up-regulate expression of drosomycin gene in Drosophila S2 cells, indicating that MsToll-MsSpz-C108 complex can activate the Toll signaling pathway. In vivo assays showed that activation of AMP genes, including cecropin, attacin, moricin and lebocin, in M. sexta larvae by purified recombinant MsSpz-C108 could be blocked by pre-injection of antibody to MsToll, further confirming a Toll-Spz pathway in M. sexta, a lepidopteran insect.
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影响因子：
4.8
作者：
HARA, S;YAMAKAWA, M
通讯作者：
YAMAKAWA, M
DOI：
10.1073/pnas.97.16.8856
发表时间：
2000-08-01
影响因子：
11.1
作者：
Hancock, REW;Scott, MG
通讯作者：
Scott, MG
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
影响因子：
4
作者：
Bettencourt, R;Asha, H;Ip, YT
通讯作者：
Ip, YT
DOI：
10.1111/j.1432-1033.1997.00614.x
发表时间：
1997-07-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Axen, A;Carlsson, A;Bennich, H
通讯作者：
Bennich, H