Beneficial effect of dietary geranylgeraniol on glucose homeostasis and bone microstructure in obese mice is associated with suppression of proinflammation and modification of gut microbiome.
Beneficial effect of dietary geranylgeraniol on glucose homeostasis and bone microstructure in obese mice is associated with suppression of proinflammation and modification of gut microbiome.
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膳食香叶基香叶醇对肥胖小鼠葡萄糖稳态和骨微结构的有益作用与抑制促炎反应和改变肠道微生物组有关。
DOI：
10.1016/j.nutres.2021.07.001
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Shen CL
中科院分区：
文献类型：
--
作者：
Chung E;Elmassry MM;Cao JJ;Kaur G;Dufour JM;Hamood AN;Shen CL
香叶醇(Geranylgeraniol，GGOH)存在于橄榄油、亚麻籽油和向日葵油等食用油中，具有良好的代谢作用。然而，目前尚不清楚这些生理益处是否通过肠道微生物群介导。因此，本研究的目的是验证补充GGOH可以通过抑制炎症和改变肠道微生物区系组成来改善肥胖小鼠的血糖稳态和有益于骨微结构的假说。将36只雄性C57BL/6J小鼠随机分为3组：低脂饲料组、高脂饲料组和高脂饲料+800 mg GGOH/kg饲料组，共14周。在基线和研究结束时测量血糖和胰岛素耐量试验。用双抗体夹心法测定血清中脂肪因子(抵抗素、瘦素、单核细胞趋化蛋白-1、白介素6)的浓度。用Micro-CT测量骨的微结构和质量。用16S rRNA扩增序列对盲肠内容物进行微生物组分析。与HFD组相比，GG组：(1)改善糖耐量和胰岛素敏感性；(2)减少促炎脂肪因子的产生；(3)增加血清I型前胶原全长N端肽(骨形成标记物)浓度，降低血清I型胶原交联型C端肽(骨吸收标记物)水平；(4)增加股骨和LV-4的硬度和股骨中段皮质厚度。与HFD组相比，GG组盲肠菌群中的乳头孢子虫丰度增加，Dorea Longicatena减少。总而言之，GGOH改善了肥胖小鼠的葡萄糖稳态和骨骼微结构，可能是通过抑制促炎和改变微生物组分来实现的。
Geranylgeraniol (GGOH) is found in edible oils such as olive, linseed, and sunflower oils, which have favorable metabolic effects. However, it is unknown whether these physiological benefits are mediated through the gut microbiome. Thus, the purpose of this study was to test the hypothesis that GGOH supplementation would improve glucose homeostasis and benefit the bone microstructure in obese mice through suppression of inflammation and modification of gut microbiota composition. Thirty-six male C57BL/6J mice were divided into 3 groups: a low-fat diet, a high-fat diet (HFD), and an HFD supplemented with 800 mg GGOH/kg diet (GG) for 14 weeks. Glucose and insulin tolerance tests were measured at baseline and end of study. The concentrations of adipokine cytokines (resistin, leptin, monocyte chemoattractant protein-1, interleukin-6) were measured via ELISA. Bone microarchitecture and quality were measured by micro-CT. Microbiome analysis was performed using 16S rRNA amplicon sequencing on cecal content. Relative to the HFD group, the GG group: (1) improved glucose tolerance and insulin sensitivity; (2) reduced production of pro-inflammatory adipokines, (3) increased serum procollagen I intact N-terminal propeptide (bone formation marker) concentrations, while decreasing serum collagen type 1 cross-linked C-telopeptide (bone resorption marker) levels, and (4) increased stiffness at both femur and LV-4 and cortical thickness at femoral midshaft. Compared to the HFD group, the GG group had an increased abundance of Butyricicoccus pullicaecorum and decreased Dorea longicatena in the cecal microbiome. Collectively, GGOH improves glucose homeostasis and bone microstructure in obese mice, probably via suppression of pro-inflammation and modification of microbiome composition.
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影响因子：
2.9
作者：
Chang SC;Shen MH;Liu CY;Pu CM;Hu JM;Huang CJ
通讯作者：
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影响因子：
8.2
作者：
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通讯作者：
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影响因子：
3.3
作者：
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通讯作者：
Hu, Jing
影响因子：
5.6
作者：
Allen, London;Ramalingam, Latha;Moustaid-Moussa, Naima
通讯作者：
Moustaid-Moussa, Naima
影响因子：
82.9
作者：
Depommier, Clara;Everard, Amandine;Cani, Patrice D.
通讯作者：
Cani, Patrice D.