A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer.
A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer.
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DOI：
10.3892/ol.2020.12190
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发表时间：
2020-12
期刊：
影响因子：
2.9
通讯作者：
Huang CJ
中科院分区：
文献类型：
--
作者：
Chang SC;Shen MH;Liu CY;Pu CM;Hu JM;Huang CJ
肠道微生物影响肿瘤在肠道中的发展和进展，并可能为结肠直肠癌（CRC）的治疗提供新的范例。肠道生态失调可能与CRC的发生和进展有关。确定结肠道和肠道微生物群之间的相互作用可以提供与CRC预防相关的新信息。研究了产丁酸盐的鸡痘丁酸球菌（B. pullicaecorum）对患有1，2-二甲基肼（DMH）诱导的CRC的小鼠和B的微生物代谢物的作用。pullicaecorum对CRC细胞的作用。采用免疫组织化学染色和逆转录PCR检测结肠癌细胞中短链脂肪酸转运蛋白溶质载体家族5成员8（SLC 5A 8）和G蛋白偶联受体43（GPR 43）的蛋白和mRNA表达水平。在喂食B的CRC荷瘤小鼠中。结果表明，DMH诱导的大肠癌消退，体重增加，血清癌胚抗原水平降低。值得注意的是，SLC 5A 8和GPR 43在小鼠结肠中的肿瘤上皮细胞和下面的固有肌层中弥漫地和中度至强烈地表达。总之，B. Pullicaecorum或其代谢物通过激活SCFA转运蛋白和/或受体改善了CRC的临床结果。这些结果表明，B. pullicaecorum是具有抗CRC潜力的益生菌。
Gut microbes influence tumor development and progression in the intestines and may provide a novel paradigm for the treatment of colorectal cancer (CRC). Gut dysbiosis may be associated with the development and progression of CRC. Identifying the interactions between the colonic tract and gut microbiota may provide novel information relevant to CRC prevention. The present study examined the effects of butyrate-producing Butyricicoccus pullicaecorum (B. pullicaecorum) on mice with 1,2-dimethylhydrazine (DMH)-induced CRC and the microbial metabolite of B. pullicaecorum on CRC cells. Immunohistochemical staining of the mouse colon tissues and reverse transcription PCR of CRC cells were used to determine the protein and mRNA expression levels of the short-chain fatty acid (SCFA) transporter solute carrier family 5 member 8 (SLC5A8) and G-protein-coupled receptor 43 (GPR43). In CRC-bearing mice fed B. pullicaecorum, DMH-induced CRC regressed, body weight increased and serum carcinoembryonic antigen levels decreased. Notably, SLC5A8 and GPR43 were diffusely and moderately to strongly expressed in the neoplastic epithelial cells and underlying muscularis propria in the colons of the mice. In conclusion, administration of B. pullicaecorum or its metabolites improved the clinical outcome of CRC by activating the SCFA transporter and/or receptor. These results indicated that B. pullicaecorum was a probiotic with anti-CRC potential.
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DOI：
10.1099/ijs.0.65730-0
发表时间：
2008-12-01
影响因子：
2.8
作者：
Eeckhaut, Venessa;Van Immerseel, Filip;Vandamme, Peter
通讯作者：
Vandamme, Peter
影响因子：
6.1
作者：
Gupta, N;Martin, PM;Ganapathy, V
通讯作者：
Ganapathy, V
影响因子：
2.7
作者：
Gui, Qifeng;Li, Hanyu;Xiao, Chi
通讯作者：
Xiao, Chi
影响因子：
10.7
作者：
Huang, Chi-Cheng;Shen, Ming-Hung;Huang, Chi-Jung
通讯作者：
Huang, Chi-Jung
影响因子：
--
作者：
Bishop KS;Xu H;Marlow G
通讯作者：
Marlow G