Genetically engineered CXCR4-modified exosomes for delivery of miR-126 mimics to macrophages alleviate periodontitis.
Genetically engineered CXCR4-modified exosomes for delivery of miR-126 mimics to macrophages alleviate periodontitis.
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基因工程 CXCR4 修饰的外泌体可将 miR-126 模拟物递送至巨噬细胞，从而缓解牙周炎。
DOI：
10.1186/s12951-023-01863-w
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发表时间：
2023-03-30
影响因子：
10.2
通讯作者：
Zhou, Chen
中科院分区：
文献类型：
--
作者：
Luo, Haotian;Chen, Danying;Li, Ruoyu;Li, Runze;Teng, Yungshan;Cao, Yang;Zou, Xuenong;Wang, Weicai;Zhou, Chen
Biofilm-related diseases are a group of diseases that tolerate antimicrobial chemotherapies and therefore are refractory to treatment. Periodontitis, a non-device chronic biofilm disease induced by dental plaque, can serve as an excellent in vivo model to study the important effects of host factors on the biofilm microenvironment. Macrophage activity is one of the key factors that modulate the progression of inflammation-driven destruction in periodontitis; therefore it is an important host immunomodulatory factor. In this study, the reduction of microRNA-126 (miR-126) with the recruitment of macrophages in periodontitis was confirmed in clinical samples, and a strategy for targeted delivery of miR-126 to macrophages was explored. Exosomes overexpressing the C-X-C motif chemokine receptor 4 (CXCR4) loaded with miR-126 (CXCR4-miR126-Exo) was successfully constructed, which reduced off-target delivery to macrophages and regulated macrophages toward the anti-inflammatory phenotype. In vivo local injection of CXCR4-miR126-Exo into sites of periodontitis in rats effectively reduced bone resorption and osteoclastogenesis and inhibited the progression of periodontitis. These results provide new insights for designing novel immunomodulatory factor targeted delivery systems to treat periodontitis and other biofilm-related diseases. The online version contains supplementary material available at 10.1186/s12951-023-01863-w.
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影响因子：
2.7
作者：
通讯作者：
--
DOI：
10.1126/science.aau6977
发表时间：
2020-02-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kalluri R;LeBleu VS
通讯作者：
LeBleu VS
影响因子：
5.6
作者：
Lin P;Niimi H;Ohsugi Y;Tsuchiya Y;Shimohira T;Komatsu K;Liu A;Shiba T;Aoki A;Iwata T;Katagiri S
通讯作者：
Katagiri S
影响因子：
5.6
作者：
Ciullo, Alessandra;Biemmi, Vanessa;Barile, Lucio
通讯作者：
Barile, Lucio
影响因子：
5.4
作者：
Edgar JR
通讯作者：
Edgar JR