Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogens.
Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity amongst closely related pathogens.
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DOI：
10.1186/1471-2164-13-545
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发表时间：
2012-10-10
期刊：
影响因子：
4.4
通讯作者：
Harvill ET
中科院分区：
文献类型：
--
作者：
Park J;Zhang Y;Buboltz AM;Zhang X;Schuster SC;Ahuja U;Liu M;Miller JF;Sebaihia M;Bentley SD;Parkhill J;Harvill ET
The classical Bordetella subspecies are phylogenetically closely related, yet differ in some of the most interesting and important characteristics of pathogens, such as host range, virulence and persistence. The compelling picture from previous comparisons of the three sequenced genomes was of genome degradation, with substantial loss of genome content (up to 24%) associated with adaptation to humans. For a more comprehensive picture of lineage evolution, we employed comparative genomic and phylogenomic analyses using seven additional diverse, newly sequenced Bordetella isolates. Genome-wide single nucleotide polymorphism (SNP) analysis supports a reevaluation of the phylogenetic relationships between the classical Bordetella subspecies, and suggests a closer link between ovine and human B. parapertussis lineages than has been previously proposed. Comparative analyses of genome content revealed that only 50% of the pan-genome is conserved in all strains, reflecting substantial diversity of genome content in these closely related pathogens that may relate to their different host ranges, virulence and persistence characteristics. Strikingly, these analyses suggest possible horizontal gene transfer (HGT) events in multiple loci encoding virulence factors, including O-antigen and pertussis toxin (Ptx). Segments of the pertussis toxin locus (ptx) and its secretion system locus (ptl) appear to have been acquired by the classical Bordetella subspecies and are divergent in different lineages, suggesting functional divergence in the classical Bordetellae. Together, these observations, especially in key virulence factors, reveal that multiple mechanisms, such as point mutations, gain or loss of genes, as well as HGTs, contribute to the substantial phenotypic diversity of these versatile subspecies in various hosts.
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影响因子：
3.1
作者：
Buboltz, Anne M.;Nicholson, Tracy L.;Harvill, Eric T.
通讯作者：
Harvill, Eric T.
影响因子：
3.1
作者：
Hausman, SZ;Cherry, JD;Burns, DL
通讯作者：
Burns, DL
DOI：
10.1126/science.1182395
发表时间：
2010-01-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Harris SR;Feil EJ;Holden MT;Quail MA;Nickerson EK;Chantratita N;Gardete S;Tavares A;Day N;Lindsay JA;Edgeworth JD;de Lencastre H;Parkhill J;Peacock SJ;Bentley SD
通讯作者：
Bentley SD
影响因子：
5.8
作者：
Carver, TJ;Rutherford, KM;Parkhill, J
通讯作者：
Parkhill, J
DOI：
10.1126/science.1180614
发表时间：
2009-10-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chain PS;Grafham DV;Fulton RS;Fitzgerald MG;Hostetler J;Muzny D;Ali J;Birren B;Bruce DC;Buhay C;Cole JR;Ding Y;Dugan S;Field D;Garrity GM;Gibbs R;Graves T;Han CS;Harrison SH;Highlander S;Hugenholtz P;Khouri HM;Kodira CD;Kolker E;Kyrpides NC;Lang D;Lapidus A;Malfatti SA;Markowitz V;Metha T;Nelson KE;Parkhill J;Pitluck S;Qin X;Read TD;Schmutz J;Sozhamannan S;Sterk P;Strausberg RL;Sutton G;Thomson NR;Tiedje JM;Weinstock G;Wollam A;Genomic Standards Consortium Human Microbiome Project Jumpstart Consortium;Detter JC
通讯作者：
Detter JC