A new approach to promote astaxanthin accumulation via Na2WO4 in Haematococcus pluvialis
A new approach to promote astaxanthin accumulation via Na2WO4 in Haematococcus pluvialis
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通过 Na2WO4 促进雨生红球藻中虾青素积累的新方法
DOI：
10.1007/s00343-018-7317-0
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发表时间：
2018-11
影响因子：
1.6
通讯作者：
Xu Nianjun
中科院分区：
文献类型：
--
作者：
Li Yanguo;Cui D;an;Zhuo Pinli;Zhang Lin;Sun Xue;Xu Nianjun
探讨了Na2WO4的最佳浓度与雨红球菌细胞密度和虾青素含量的关系。测定细胞形态、硝酸还原酶（NR）活性、可溶性糖和蛋白含量、叶绿素荧光，并采用实时荧光定量PCR技术检测致胡萝卜素基因的转录表达。Na2WO4处理后，NR活性显著且持续下降。试验期间可溶性糖含量逐渐升高，最终显著高于对照。可溶性蛋白含量迅速增加，在第0.5天和第1天达到最大值，然后下降。有效光化学效率PSII （Fv'/Fm'）和光饱和度（Ek）先下降后趋于稳定，NADP+-甘油醛-3-磷酸脱氢酶（GAPDH）基因表达与光合作用相关。施用Na2WO4后，ipi、psy和bkt的转录表达量较对照显著增加，其中ipi的相对表达量在第5天达到最高水平，增加了98.03±1.92倍。我们的研究结果描述了利用NR抑制剂Na2WO4促进雨水杨虾青素有效积累的新途径。
The optimum concentration of Na2WO4 was explored in relation to the cell density and astaxanthin content in Haematococcus pluvialis. Then, the cellular morphology, nitrate reductase (NR) activity, soluble sugar and protein contents, and chlorophyll fluorescence were measured, and the transcriptional expression of carotenogenic genes was determined by quantitative real-time PCR. The NR activity decreased significantly and continually after Na2WO4 treatment. The soluble sugar content increased gradually during the experimental period and was eventually significantly higher than that in the control. The soluble protein content increased rapidly, reached a maximum in day 0.5 and day 1 and then decreased. The effective photochemical efficiency of PSII ( Fv'/Fm') and light saturation ( Ek) first decreased and then tended to stabilize, and NADP+-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression was correlated with photosynthesis. The transcriptional expression of ipi, psy and bkt was significantly increased compared with that in the control after application of Na2WO4, and the relative expression of ipi reached the highest level on the fifth day, with a 98.03±1.92-fold increase. Our results describe a new approach to promote the effective accumulation of astaxanthin in H. pluvialis by NR inhibitor Na2WO4.
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影响因子：
7.4
作者：
Q. Wei
通讯作者：
Q. Wei
影响因子：
4.5
作者：
Barbosa, MJ;Morais, R;Choubert, G
通讯作者：
Choubert, G
影响因子：
15
作者：
Wouters, J;Oudjama, Y;Oldfield, E
通讯作者：
Oldfield, E
影响因子：
3.1
作者：
EILERS, PHC;PEETERS, JCH
通讯作者：
PEETERS, JCH
DOI：
--
发表时间：
2014-11
期刊：
--
影响因子：
--
作者：
A. A
通讯作者：
A. A