Synthetic miRNAs induce dual arboviral-resistance phenotypes in the vector mosquito Aedes aegypti.
Synthetic miRNAs induce dual arboviral-resistance phenotypes in the vector mosquito Aedes aegypti.
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DOI：
10.1038/s42003-017-0011-5
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发表时间：
2018
影响因子：
5.9
通讯作者：
Failloux AB
中科院分区：
文献类型：
--
作者：
Yen PS;James A;Li JC;Chen CH;Failloux AB
蚊子传播的虫媒病毒是最近登革热、基孔肯雅热和寨卡大流行的原因。黄热病蚊子埃及伊蚊在这三种病毒的传播中起着重要作用。我们开发了一种基于miRNA的方法，该方法导致蚊子对登革热血清型3（DENV-3）和基孔肯雅（CHIKV）病毒的双重抗性表型。靶病毒来自两个不同的虫媒病毒家族，抗病毒机制被设计为通过感染蚊子的内源性miRNA途径发挥作用。攻击实验表明转基因蚊子的病毒传播效率降低。蚊子健身的几个组成部分进行了检查，与PUb启动子的转基因蚊子在所有发展阶段表现出轻微的健身成本。利用基因编辑工具进一步开发这些毒株，可以使它们成为可持续控制多种虫媒病毒疾病的种群替代战略的候选者。Pei-Shi Yen等报道了一种工程化转基因埃及伊蚊以表达同时靶向两种不同虫媒病毒登革热和基孔肯雅病毒的抗病毒miRNA的策略。该策略导致在受感染的血餐后减少蚊子唾液中的病毒传播。
Mosquito-borne arboviruses are responsible for recent dengue, chikungunya, and Zika pandemics. The yellow-fever mosquito, Aedes aegypti, plays an important role in the transmission of all three viruses. We developed a miRNA-based approach that results in a dual resistance phenotype in mosquitoes to dengue serotype 3 (DENV-3) and chikungunya (CHIKV) viruses. The target viruses are from two distinct arboviral families and the antiviral mechanism is designed to function through the endogenous miRNA pathway in infected mosquitoes. Challenge experiments showed reductions in viral transmission efficiency of transgenic mosquitoes. Several components of mosquito fitness were examined, and transgenic mosquitoes with the PUb promoter showed minor fitness costs at all developing stages. Further development of these strains with gene editing tools could make them candidates for releases in population replacement strategies for sustainable control of multiple arbovirus diseases. Pei-Shi Yen et al. report a strategy for engineering transgenic Aedes aegypti mosquitoes to express antiviral miRNAs that simultaneously target two distinct arboviruses, dengue and chikungunya. The strategy results in reduced viral transmission in the mosquito saliva following an infected bloodmeal.
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影响因子：
3.7
作者：
Carter JR;Taylor S;Fraser TS;Kucharski CA;Dawson JL;Fraser MJ Jr
通讯作者：
Fraser MJ Jr
影响因子：
3.8
作者：
Franz AW;Sanchez-Vargas I;Raban RR;Black WC 4th;James AA;Olson KE
通讯作者：
Olson KE
影响因子：
64.5
作者：
Foerstemann, Klaus;Horwich, Michael D.;Zamore, Phillip D.
通讯作者：
Zamore, Phillip D.
影响因子：
4
作者：
Joga MR;Zotti MJ;Smagghe G;Christiaens O
通讯作者：
Christiaens O
DOI：
10.1073/pnas.1521077112
发表时间：
2015-12-08
影响因子：
11.1
作者：
Gantz, Valentino M.;Jasinskiene, Nijole;James, Anthony A.
通讯作者：
James, Anthony A.