RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far.
RNAi Efficiency, Systemic Properties, and Novel Delivery Methods for Pest Insect Control: What We Know So Far.
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DOI：
10.3389/fphys.2016.00553
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发表时间：
2016
影响因子：
4
通讯作者：
Christiaens O
中科院分区：
文献类型：
--
作者：
Joga MR;Zotti MJ;Smagghe G;Christiaens O
近年来，利用RNA干扰技术抑制作物害虫的研究取得了显著的发展。然而，考虑到在许多昆虫中观察到的RNAi效率的可变性，使用RNAi的昆虫害虫管理的新方法的开发首先需要解开dsRNA介导的基因沉默效率背后的因素。在这篇综述中，我们探讨了通过非转化方法递送dsRNA来提高RNAi效率的重要意义和可能性。我们讨论了影响昆虫RNAi机制的因素和dsRNA的系统特性。最后，新的策略，提供dsRNA进行了讨论，包括共生体，植物病毒，树干注射，根浸泡，和transplastomic植物。
In recent years, the research on the potential of using RNA interference (RNAi) to suppress crop pests has made an outstanding growth. However, given the variability of RNAi efficiency that is observed in many insects, the development of novel approaches toward insect pest management using RNAi requires first to unravel factors behind the efficiency of dsRNA-mediated gene silencing. In this review, we explore essential implications and possibilities to increase RNAi efficiency by delivery of dsRNA through non-transformative methods. We discuss factors influencing the RNAi mechanism in insects and systemic properties of dsRNA. Finally, novel strategies to deliver dsRNA are discussed, including delivery by symbionts, plant viruses, trunk injections, root soaking, and transplastomic plants.
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影响因子：
3.8
作者：
Arimatsu, Yuji;Kotani, Eiji;Furusawa, Toshiharu
通讯作者：
Furusawa, Toshiharu
影响因子：
2.2
作者：
Allen, Margaret L.;Walker, William B., III
通讯作者：
Walker, William B., III
影响因子：
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影响因子：
4.6
作者：
Abd El Halim HM;Alshukri BM;Ahmad MS;Nakasu EY;Awwad MH;Salama EM;Gatehouse AM;Edwards MG
通讯作者：
Edwards MG
影响因子：
3.7
作者：
Berry B;Deddouche S;Kirschner D;Imler JL;Antoniewski C
通讯作者：
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