Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens.
Rosemary Reduces Heat Stress by Inducing CRYAB and HSP70 Expression in Broiler Chickens.
复制标题
迷迭香通过诱导肉鸡 CRYAB 和 HSP70 表达来减轻热应激。
DOI：
10.1155/2018/7014126
复制
发表时间：
2018
影响因子：
--
通讯作者：
Bao E
中科院分区：
文献类型：
--
作者：
Tang S;Yin B;Xu J;Bao E
Heat stress negatively affects poultry production and animal health. In response, animals invoke a heat stress response by inducing heat shock proteins (HSPs). Scientists are actively seeking natural products that can enhance the heat shock response. The present study aimed at assessing the effects of a purified rosemary extract comprising antioxidant compounds on the heat shock response and HSP expression profile in broiler chickens. The response of broilers to HS in the presence of purified rosemary extract was assessed using an in vivo myocardial cell model. Pathological lesions of heart tissue were examined microscopically. The levels and activities of enzymes associated with heart damage and oxidative damage were detected. Immunohistochemical staining was performed for HSPs in myocardial cells. The results showed that lactate dehydrogenase (LDH), creatine kinase (CK), and myocardial CK (CKMB) levels were reduced by the purified rosemary extract before and during heat stress. Heat stress alone increased CK and CKMB levels. The levels of oxidative damage-associated enzymes were compared between the rosemary + heat stress and heat stress-alone groups. The results indicated that in terms of these enzymes, the purified rosemary extract induced a more antioxidative state. Pathological examinations showed that heat stress caused myocardial fiber fracture, karyopyknosis, and degeneration. The addition of purified rosemary extract ameliorated these lesions to some degree, preserving more of the basic structure. Heat stress decreased the cellular levels of crystallin alpha B (CRYAB) and HSP70. The addition of the purified rosemary extract significantly increased the levels of CRYAB and HSP70 during heat stress (p < 0.0001). Immunohistochemistry showed that after rosemary treatment, CRYAB and HSP70 showed more intense staining compared with the no heat stress control group. In the rosemary + heat group, after 10 hours of heat stress, the staining intensity of these two proteins remained higher than in the heat stress group. Thus, purified rosemary extract could induce high levels of HSP70 and CRYAB in chicken hearts before and during heat stress. Purified rosemary extract could be used to alleviate heat stress in broiler chickens.
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影响因子：
11.4
作者：
Jäättelä, M;Wissing, D;Egeblad, M
通讯作者：
Egeblad, M
影响因子：
6.1
作者：
Hopia, AI;Huang, SW;Frankel, EN
通讯作者：
Frankel, EN
影响因子：
--
作者：
Hamilton TR;Mendes CM;de Castro LS;de Assis PM;Siqueira AF;Delgado Jde C;Goissis MD;Muiño-Blanco T;Cebrián-Pérez JÁ;Nichi M;Visintin JA;Assumpção ME
通讯作者：
Assumpção ME
DOI：
10.1016/j.biocel.2012.04.002
发表时间：
2012-10-01
影响因子：
4
作者：
Acunzo, Julie;Katsogiannou, Maria;Rocchi, Palma
通讯作者：
Rocchi, Palma
影响因子：
3.8
作者：
Birtic, Simona;Dussort, Pierre;Roller, Marc
通讯作者：
Roller, Marc