Pyrene-based quantitative detection of the 5-formylcytosine loci symmetry in the CpG duplex content during TET-dependent demethylation.
Pyrene-based quantitative detection of the 5-formylcytosine loci symmetry in the CpG duplex content during TET-dependent demethylation.
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DOI：
10.1002/anie.201406220
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发表时间：
2014-10-13
影响因子：
16.6
通讯作者：
Wang, Dong
中科院分区：
文献类型：
--
作者：
Xu, Liang;Chen, Ying-Chu;Chong, Jenny;Fin, Andrea;McCoy, Lisa S.;Xu, Jun;Zhang, Chao;Wang, Dong
5-甲基胞嘧啶（5mC）在CpG岛中多呈对称分布。10 - 11易位（TET）蛋白是通过5mC的逐步氧化参与活性DNA去甲基化的关键酶。然而，对称甲基化CpG背景下TET酶的氧化途径仍然是难以捉摸的。利用芘基团独特的荧光特性，我们设计并合成了一种灵敏的荧光探针，不仅可以靶向5-甲酰基胞嘧啶（5fC）位点，还可以在et依赖的5mC氧化过程中区分双链DNA背景下的对称和不对称5fC位点。利用这种新型探针，我们揭示了体外tet依赖性5mC氧化过程中所有5fC位点中对称5fC的优势水平，并对mCpG背景下tet依赖性5mC氧化的机制有了新的认识。
5-Methylcytosine (5mC) is mostly symmetrically distributed in CpG islands. Ten-eleven-translocation (TET) proteins are the key enzymes involved in active DNA demethylation through stepwise oxidation of 5mC. However, oxidation pathways of TET enzymes in the symmetrically methylated CpG context are still elusive. Employing the unique fluorescence properties of pyrene group, we designed and synthesized a sensitive fluorescence-based probe not only to target 5-formylcytosine (5fC) sites, but also to distinguish symmetric from asymmetric 5fC sites in the double stranded DNA context during TET-dependent 5mC oxidation process. Using this novel probe, we revealed dominant levels of symmetric 5fC among total 5fC sites during in vitro TET-dependent 5mC oxidation and novel mechanistic insights into the TET-dependent 5mC oxidation in the mCpG context.
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影响因子：
64.5
作者：
Bhutani N;Burns DM;Blau HM
通讯作者：
Blau HM
影响因子：
21.8
作者：
通讯作者：
--
影响因子：
4.8
作者：
Maiti, Atanu;Drohat, Alexander C.
通讯作者：
Drohat, Alexander C.
哺乳动物 DNA 中 Tet 介导的 5-羧基胞嘧啶的形成及其 TDG 的切除
DOI：
10.1126/science.1210944
发表时间：
2011-09-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
He YF;Li BZ;Li Z;Liu P;Wang Y;Tang Q;Ding J;Jia Y;Chen Z;Li L;Sun Y;Li X;Dai Q;Song CX;Zhang K;He C;Xu GL
通讯作者：
Xu GL
影响因子：
5.2
作者：
Guo, Pu;Yan, Shengyong;Zhou, Xiang
通讯作者：
Zhou, Xiang