Light-induced asymmetries in the embryonic retina are mediated by the vascular system and extracellular matrix
Light-induced asymmetries in the embryonic retina are mediated by the vascular system and extracellular matrix
复制标题
胚胎视网膜中光引起的不对称是由血管系统和细胞外基质介导的
DOI：
10.1101/2021.10.08.463575
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Versace E
中科院分区：
文献类型：
--
作者：
Versace E
神经系统中的左右不对称（偏侧化）影响着广泛的行为，从社交反应到导航和语言。内源性和环境机制在侧化个体发育中的作用和途径仍有待建立。国内小鸡是一个模型的内源性和经验诱导的偏侧化驱动的曝光。随着胚胎的内源性旋转，卵子中的不对称位置导致右眼更多地暴露于环境光。为了确定由不对称光刺激激活的遗传途径及其时间过程，我们将胚胎暴露于不同的光照条件下：黑暗，6小时光照和24小时光照。我们使用RNA-seq来比较左右视网膜和端脑中的基因表达。正如预期的那样，在黑暗中不存在左右之间的差异表达。我们检测到差异基因表达的权利与左视网膜曝光后6小时。这种差异在光照24小时前消失，表明光诱导的激活是一种自终止现象。这种短暂的光暴露效应与右视网膜中敏感期介质基因DIO 2（碘甲腺原氨酸脱碘酶2）的下调有关。在右侧与左侧端脑中表达的基因之间没有检测到差异。与偏侧化相关的基因网络与血管化、细胞运动和细胞外基质有关。有趣的是，我们知道细胞外基质-包括差异表达的PDGFRB（血小板衍生生长因子受体β）基因-参与敏感期和初级纤毛的内源性手性机制，驱动侧化。我们的数据显示了内源性和经验驱动的侧化之间的相似性，确定了在特定时间窗口内影响侧化的功能基因网络。
Left-right asymmetries in the nervous system (lateralisation) influence a broad range of behaviours, from social responses to navigation and language. The role and pathways of endogenous and environmental mechanisms in the ontogeny of lateralisation remains to be established. The domestic chick is a model of both endogenous and experience-induced lateralisation driven by light exposure. Following the endogenous rightward rotation of the embryo, the asymmetrical position in the egg results in a greater exposure of the right eye to environmental light. To identify the genetic pathways activated by asymmetric light stimulation, and their time course, we exposed embryos to different light regimes: darkness, 6 hours of light and 24 hours of light. We used RNA-seq to compare gene expression in the right and left retinas and telencephalon. As expected, no differential expression between left and right was present in darkness. We detected differential gene expression in right vs left retina after 6 hours of light exposure. This difference disappeared before 24 hours of light exposure, suggesting that light-induced activation is a self-terminating phenomenon. This transient effect of light exposure was associated with a downregulation of the sensitive-period mediator gene DIO2 (iodothyronine deiodinase 2) in the right retina. No differences between genes expressed in the right vs. left telencephalon were detected. Gene networks associated with lateralisation were connected to vascularisation, cell motility, and the extracellular matrix. Interestingly, we know that the extracellular matrix– including the differentially expressedPDGFRB(platelet-derived growth factor receptor β) gene – is involved in both sensitive periods and in the endogenous chiral mechanism of primary cilia, that drives lateralisation. Our data show a similarity between endogenous and experience-driven lateralisation, identifying functional gene networks that affect lateralisation in a specific time window.
登录
查看更多内容
影响因子：
2.7
作者：
L. Rogers;C. Deng
通讯作者：
C. Deng
影响因子：
9.2
作者：
Barth, KA;Miklosi, A;Watkins, J;Bianco, IH;Wilson, SW;Andrew, RJ
通讯作者：
Andrew, RJ
鸡胚胎的光照影响印记记忆的侧化回忆。
影响因子：
1.9
作者：
A. Johnston;L. Rogers
通讯作者：
L. Rogers
鸡的性别差异和睾酮对大脑不对称性的逆转
影响因子：
2.7
作者：
J. Zappia;L. Rogers
通讯作者：
L. Rogers
影响因子：
3.8
作者：
Correia JM;Jansma B;Hausfeld L;Kikkert S;Bonte M
通讯作者：
Bonte M