A Quantitative Polymerase Chain Reaction Study-猫眼课题宝

定量聚合酶链式反应研究

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发表时间：

2004

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作者：

J. Kira;Y. Koyanagi;Takeshi Yamada;Y. Itoyama;I. Goto;Naoki Yamamoto;H. Sasaki;Y. Sakaki

应用聚合酶链式反应技术，对18例HTLV-I相关性脊髓性痉挛截瘫患者、17例无HTLV-I相关性脊髓病/热带痉挛麻痹患者、伴或不伴其他自身免疫性或炎症性疾病的HTLV-I携带者和19例血清阴性对照的外周血单核细胞进行HTLV-I前病毒DNA定量检测。HTLV-I前病毒DNA在有自身免疫性疾病或炎症性疾病的患者和无HAM/TSP的HTLV-I携带者中比无自身免疫性或炎症性疾病的携带者高10~100倍。起病年龄较小(15~39岁)的患者早期HTLV-I前病毒DNA水平极高，而起病较晚(44~61岁)患者的HTLV-I前病毒DNA水平极高。外周血单核细胞中HTLV-I前病毒DNA的大量增加可能与HTLV-I携带者自身免疫或炎症过程的发展密切相关，包括HTLV-I相关性肾小球疾病/热带痉挛截瘫。

Using the polymerase chain reaction, we quantitated the amount of human T-lymphotropic virus type I (HTLV-I) proviral DNA in peripheral blood mononuclear cells from 18 patients with HTLV-I-associated myelopathyitropical spastic paraparesis; 17 HTLV-I carriers without HTLV-I-associated myelopathy/tropical spastic paraparesis, with or without other autoimmune or inflammatory diseases; and 19 seronegative control subjects. The HTLV-I proviral DNA was 10to 100-fold higher in the patients and in the HTLV-I carriers without HAM/TSP who had autoimmune or inflammatory diseases than in the carriers without autoimmune or inflammatory diseases. The patients who had had onset of myelopathy at a younger age (15 to 39 years) had an extremely high level of HTLV-I proviral DNA in the early phase, as compared with findings in those with a late onset of myelopathy (at 44 to 61 years). The large increase in HTLV-I proviral DNA in peripheral blood mononuclear cells is presumably closely related to the development of autoimmune or inflammatory processes in HTLV-I carriers, including HTLV-I-associated rnyelopathy/tropical spastic paraparesis.

Detection of sequences homologous to human retroviral DNA in multiple sclerosis by gene amplification.