Effect of temperature on endplate potential rundown and recovery in rat diaphragm.
Effect of temperature on endplate potential rundown and recovery in rat diaphragm.
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温度对大鼠膈肌终板电位下降和恢复的影响。
DOI：
10.1152/jn.2001.85.5.2070
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
vanLunteren,E
中科院分区：
文献类型：
--
作者：
Moyer,M;vanLunteren,E
神经肌肉接头终板电位(EPPS)的波幅在列车内迅速下降，但在间歇刺激时几乎完全恢复。已经证明，衰减率不仅取决于递质释放的速度，还取决于释放部位耗尽的神经递质的补充速度。已经提出了两组突触小泡回收的过程，这两组过程都涉及多个需要能量的步骤和酶反应，因此预计这将是非常温度敏感的。目前的研究验证了这样的假设，即低温因此增加了EPP幅度下降的速度。在大鼠横隔膜上进行了体外研究，并使用μ-芋螺毒素允许从完整的纤维中记录正常大小的EPPS。在20°C和50°C(占空比333ms)的间歇刺激期间，EPP幅度下降在20°C时比在37°C时更大。最初，温度只影响列车内下降，但在较长时间的刺激下，寒冷温度显著加速了列车内和列车间下降。通过连续相加刺激期间每个EPP的幅度来计算累积的EPP幅度，以提供神经肌肉接头的总神经递质释放的估计。在20和50赫兹刺激下，20℃时的EPP累积幅度显著低于37℃时的EPP累积幅度。这些数据表明，EPP幅值衰减和恢复的机制是温度敏感的，冷时EPP幅值的下降幅度大于暖温。
The amplitude of neuromuscular junction end-plate potentials (EPPs) decreases quickly within a train but recovers nearly completely from train to train during intermittent stimulation. Rundown has been shown to be dependent not only on the rate of transmitter release but also on the rate of replenishment of the depleted neurotransmitter at the site of release. Two groups of processes have been proposed for synaptic vesicle recycling, both of which involve multiple energy-requiring steps and enzymatic reactions and which therefore would be expected to be very temperature-sensitive. The present study tested the hypothesis that low temperature therefore increases the rate of EPP amplitude rundown. Studies were performed in vitro on rat diaphragm and used μ-conotoxin to allow normal-sized EPPs to be recorded from intact fibers. EPP amplitude rundown during intermittent stimulation at 20 and 50 Hz (duty cycle 333 ms) was greater at 20°C than it was at 37°C. Initially, temperature affected only intra-train rundown but, over longer periods of stimulation, both intra- and inter-train rundown were significantly accelerated by cold temperature. Cumulative EPP amplitudes were calculated by successively adding the amplitudes of each EPP during the stimulation period to provide an estimate of total neurotransmitter release in the neuromuscular junction. The cumulative EPP amplitude was significantly lower at 20°C than it was at 37°C during both 20 and 50 Hz stimulation. These data indicate that the mechanism involved in EPP amplitude rundown and recovery is temperature-sensitive, with a greater decrement in EPP amplitude at cold than at warm temperatures.
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DOI：
10.1016/0165-3806(94)90317-4
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