Diagnostic Accuracy of Cell Block Preparations and Clinical Features Affecting It in Vitreoretinal Lymphoma.
Diagnostic Accuracy of Cell Block Preparations and Clinical Features Affecting It in Vitreoretinal Lymphoma.
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DOI：
10.3390/jcm11051391
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发表时间：
2022-03-03
影响因子：
3.9
通讯作者：
Ishida S
中科院分区：
文献类型：
--
作者：
Kase S;Namba K;Iwata D;Mizuuchi K;Suzuki K;Ito T;Hase K;Kitaichi N;Ishida S
目的：本研究的目的是检查玻璃体视网膜淋巴瘤（VRL）患者的细胞块（CB）方法的诊断准确性和影响它的临床特征。方法：对33例38眼VRL患者和7例7眼特发性葡萄膜炎患者行诊断性玻璃体切割术。回顾性检索包括CB细胞学、白细胞介素（IL）-10/-6浓度和免疫球蛋白重链基因（IgH）重排结果在内的病历。结果：VRL患者包括16名女性和17名男性，发病年龄为44至85岁（平均：70岁）。CB标本在35只眼（92%）中检测到大的恶性细胞，而其他3只VRL眼为阴性。后3只眼中有2只眼视网膜下浸润，CB阳性35只眼中有7只眼存在视网膜下浸润。玻璃体内IL-10和IL-6浓度分别为1866 ± 4088 pg/mL和98 ± 139 pg/mL，IL-10/-6 >1的比例为86.9%。IgH单克隆抗体阳性率为63.2%。在葡萄膜炎患者中，CB标本显示无非典型但小的炎性细胞。IL-6浓度为311.1 ± 240 pg/mL，IL-10在6只眼中检测不到，IL-10阴性率为85.7%。葡萄膜炎6眼（85.7%）IgH无单克隆抗体阳性。结论：VRL中CB制备物的诊断准确性可以达到与IL比率计算和IgH单克隆检测相当的结果。视网膜下浸润的出现可降低CB阳性率。
Purpose: The purpose of this study was to examine the diagnostic accuracy of the cell block (CB) method and clinical features affecting it in patients with vitreoretinal lymphoma (VRL). Methods: This study enrolled 38 eyes in 33 VRL patients, and 7 eyes in 7 patients with idiopathic uveitis who underwent diagnostic vitrectomy. Medical records including the results of CB cytology, interleukin (IL)-10/-6 concentrations, and immunoglobulin heavy chain gene (IgH) rearrangement were retrospectively searched. Results: Patients with VRL comprised 16 women and 17 men, and the age of onset ranged from 44 to 85 years (mean: 70 years). CB preparations detected large malignant cells in 35 eyes (92%), whereas the other 3 VRL eyes were negative. Two of the latter three eyes showed subretinal infiltrates, which existed in 7 of 35 CB-positive eyes. Intravitreal IL-10 and -6 concentrations were 1866 ± 4088 pg/mL and 98 ± 139 pg/mL, respectively, and the rate of IL-10/-6 >1 was 86.9%. The presence of IgH monoclonality was 63.2%. In patients with uveitis, CB specimens revealed no atypical but small inflammatory cells. IL-6 concentration was 311.1 ± 240 pg/mL, whereas IL-10 was undetectable in six eyes, and the IL-negative rate was 85.7%. Six eyes (85.7%) with uveitis showed no IgH monoclonality. Conclusions: Diagnostic accuracy of CB preparations in VRL could achieve an equivalent outcome to IL ratio calculation and IgH monoclonality detection. The appearance of subretinal infiltrates may diminish the CB positivity.
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影响因子：
2.6
作者：
Kase S;Namba K;Iwata D;Mizuuchi K;Kitaichi N;Tagawa Y;Okada-Kanno H;Matsuno Y;Ishida S
通讯作者：
Ishida S
影响因子：
8.1
作者：
Hiemcke-Jiwa, Laura S.;Ten Darn-van Loon, Ninette H.;Kuiper, Jonas J. W.
通讯作者：
Kuiper, Jonas J. W.
DOI：
10.1097/iae.0000000000002319
发表时间：
2019-04-01
影响因子：
3.3
作者：
Miserocchi, Elisabetta;Ferreri, Andres J. M.;Modorati, Giulio M.
通讯作者：
Modorati, Giulio M.
影响因子：
3.9
作者：
Intzedy, L.;Teoh, S. C. B.;Pawade, J.
通讯作者：
Pawade, J.
影响因子：
2
作者：
Zhao, Huiying;Wang, Xiaona;Peng, Xiaoyan
通讯作者：
Peng, Xiaoyan