cDNA cloning and expression analysis of a hepcidin gene from yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)
cDNA cloning and expression analysis of a hepcidin gene from yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)
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黄颡鱼铁调素基因的 cDNA 克隆及表达分析
DOI：
10.1016/j.fsi.2016.10.049
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发表时间：
2017
影响因子：
4.7
通讯作者：
Tang Bo-Ping
中科院分区：
文献类型：
--
作者：
Liu Qiu-Ning;Xin Zhao-Zhe;Zhang Dai-Zhen;Jiang Sen-Hao;Chai Xin-Yue;Wang Zheng-Fei;Li Chao-Feng;Zhou Chun-Lin;Tang Bo-Ping
铁调素是一种富含半胱氨酸的小分子抗菌肽，具有高度保守的β折叠结构，在宿主对病原体的先天免疫中起着至关重要的作用。本研究从我国重要的淡水鱼类黄颡鱼（Pelteobagrus fulvidraco）中克隆到一个hepcidin基因。该基因被命名为PfHep。完整的PfHepcDNA全长723 bp，包括102 bp的5 '非翻译区（UTR）、339 bp的3'非翻译区（UTR）和282 bp的开放阅读框，编码93个氨基酸的多肽，其中包括预测的信号肽和Hepcidin结构域。预测的成熟阳离子PfHep蛋白具有典型的铁调素RX（K/R）R基序和8个保守的半胱氨酸残基。推导的PfHep蛋白序列与斑点叉尾鱼、斑马鱼和智人的hepcidin蛋白的同源性分别为70%、54%和39%。PfHep的预测三级结构与其他动物中的铁调素非常相似。系统进化分析表明PfHep与斑点叉尾刺和叉尾刺的hepcidin基因有很近的亲缘关系。实时荧光定量RT-PCR结果表明，PfHepmRNA在健康黄颡鱼肝脏中表达最多，在所有组织中均有一定程度的表达。分别用脂多糖和多聚核糖肌苷酸：多聚核糖胞苷酸（poly I：C）攻击后，PfHeps在肝、脾、头肾和血液中的表达水平在不同时间点均显著上调。这些结果表明PfHep可能是先天免疫系统的重要组成部分，并参与对入侵病原体的免疫防御。
Hepcidin is a small, cysteine-rich antimicrobial peptide with a highly conserved β-sheet structure that plays a vital role in innate host immunity against pathogenic organisms. In this study, a hepcidin gene was identified inPelteobagrus fulvidraco, an economically important freshwater fish in China. The gene is namedPfHep. The completePfHepcDNA was 723 bp, including a 5'-untranslated region (UTR) of 102 bp, a 3′-UTR of 339 bp and an open reading frame of 282 bp encoding a polypeptide of 93 amino acids, which includes a predicted signal peptide and the Hepcidin domain. The predicted mature, cationic PfHep protein has a typical hepcidin RX (K/R)R motif and eight conserved cysteine residues. The deduced PfHep protein sequence has 70%, 54% and 39% percent identity with hepcidins fromIctalurus punctatus,Danio rerio, andHomo sapiens, respectively. The predicted tertiary structure of PfHep is very similar to that of hepcidin in other animals. Phylogenetic analysis revealed that PfHep is closely related to the hepcidins ofI. punctatusandI. furcatus. Real-time quantitative reverse transcription-PCR showed that thePfHepgene was expressed most in liver of healthyP. fulvidraco, and expressed to some extent in all the tissues tested. After challenge with lipopolysaccharide and polyriboinosinic:polyribocytidylic acid (poly I:C), respectively, the expression levels ofPfHepwere markedly upregulated in liver, spleen, head kidney and blood at different time points. Together these results imply that PfHep may be an important component of the innate immune system and be involved in immune defense against invading pathogens.
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影响因子：
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作者：
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