Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses.
Distinct summer and winter bacterial communities in the active layer of Svalbard permafrost revealed by DNA- and RNA-based analyses.
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DOI：
10.3389/fmicb.2015.00399
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发表时间：
2015
影响因子：
5.2
通讯作者：
Priemé A
中科院分区：
文献类型：
--
作者：
Schostag M;Stibal M;Jacobsen CS;Bælum J;Taş N;Elberling B;Jansson JK;Semenchuk P;Priemé A
北极永久冻土层上的土壤活性层每年都会遭受温度和土壤化学的巨大变化，这可能会影响细菌活动和群落结构。我们通过从一年中每月收集的 12 个土壤核心中共同提取 DNA 和 RNA，研究了斯瓦尔巴群岛（北纬 78°）活性层土壤细菌群落的季节变化。对 16S rRNA 基因 (DNA) 和逆转录本 (cDNA) 的 PCR 扩增子进行定量和测序，以测试冬季低温和易降解有机物浓度的季节变化对细菌群落的影响。 16S rRNA 基因和转录本的拷贝数显示，尽管土壤温度远低于 -10°C，但没有明显的季节变化，表明冬季潜在的细菌活动。对细菌多样性数据（DNA 和 cDNA 文库）的多变量统计分析揭示了样本的季节聚类，例如，潜在活性蓝藻的相对丰度在 6 月达到峰值，而 Alphaproteobacteria 在夏季增加，然后从 10 月到 11 月下降。主体（基于 DNA）群落的结构与 pH 值和溶解有机碳显着相关，而潜在活性（基于 RNA）群落结构与任何测量的土壤参数均不显着相关。 16S rRNA 转录本的很大一部分被分配给固氮细菌（6 月份高达 24%）和光养生物（6 月份高达 48%），说明固氮作用在氮贫的北极生态系统中以及土壤表面光养细菌活动的潜在重要性。
The active layer of soil overlaying permafrost in the Arctic is subjected to dramatic annual changes in temperature and soil chemistry, which likely affect bacterial activity and community structure. We studied seasonal variations in the bacterial community of active layer soil from Svalbard (78°N) by co-extracting DNA and RNA from 12 soil cores collected monthly over a year. PCR amplicons of 16S rRNA genes (DNA) and reverse transcribed transcripts (cDNA) were quantified and sequenced to test for the effect of low winter temperature and seasonal variation in concentration of easily degradable organic matter on the bacterial communities. The copy number of 16S rRNA genes and transcripts revealed no distinct seasonal changes indicating potential bacterial activity during winter despite soil temperatures well below −10°C. Multivariate statistical analysis of the bacterial diversity data (DNA and cDNA libraries) revealed a season-based clustering of the samples, and, e.g., the relative abundance of potentially active Cyanobacteria peaked in June and Alphaproteobacteria increased over the summer and then declined from October to November. The structure of the bulk (DNA-based) community was significantly correlated with pH and dissolved organic carbon, while the potentially active (RNA-based) community structure was not significantly correlated with any of the measured soil parameters. A large fraction of the 16S rRNA transcripts was assigned to nitrogen-fixing bacteria (up to 24% in June) and phototrophic organisms (up to 48% in June) illustrating the potential importance of nitrogen fixation in otherwise nitrogen poor Arctic ecosystems and of phototrophic bacterial activity on the soil surface.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
64.8
作者：
DeConto, Robert M.;Galeotti, Simone;Beerling, David J.
通讯作者：
Beerling, David J.
影响因子：
11
作者：
Blazewicz, Steven J.;Barnard, Romain L.;Firestone, Mary K.
通讯作者：
Firestone, Mary K.
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1099/ijs.0.037663-0
发表时间：
2012-12-01
影响因子：
2.8
作者：
Chakravarthy, S. Kalyana;Ramaprasad, E. V. V.;Ramana, Ch. V.
通讯作者：
Ramana, Ch. V.