Fermentation Process and Metabolic Flux of Ethanol Production from the Detoxified Hydrolyzate of Cassava Residue.
Fermentation Process and Metabolic Flux of Ethanol Production from the Detoxified Hydrolyzate of Cassava Residue.
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木薯渣解毒水解液生产乙醇的发酵过程和代谢通量。
DOI：
10.3389/fmicb.2017.01603
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Li X;Deng Y;Yang Y;Wei Z;Cheng J;Cao L;Mu D;Luo S;Zheng Z;Jiang S;Wu X
随着世界人口的增长，能源问题日益严重，因此，可持续能源变得非常重要。在乙醇燃料生产方面，生物质正逐步取代谷物成为主要原料。在这项研究中，我们探索了五碳和六碳糖，主要的生物质降解产物，发酵成酒精。我们专门针对热带假丝酵母CICC 1779进行了诱变筛选，以获得有效利用木糖的菌株（热带假丝酵母CICC 1779-Dyd）。通过研究不同初始液体体积氧传递系数（kLα）下的发酵条件，并在最佳条件下耦合控制通气量和搅拌速度，得到最佳溶解氧变化曲线。此外，我们还构建了代谢流程图和代谢方程，以更好地了解发酵机理，提高乙醇产量。在我们的实验中，野生型菌株在kLα为120时的乙醇产量为17.58 g·L−1。诱变菌株的最高乙醇产量为24.85 g·L-1。当溶解氧含量优化时，乙醇产量增加到26.56 g·L-1，糖转化为乙醇的转化率达到0.447 g·g-1葡萄糖（酵母代谢木糖的理论效价为0.46 g乙醇/g木糖，葡萄糖乙醇发酵效价为0.51 g乙醇/g葡萄糖）。最后，木糖还原酶和木糖脱氢酶的活性检测结果表明，突变株CICC 1779-Dyd能够有效地利用木糖进行代谢。
With the growth of the world population, energy problems are becoming increasingly severe; therefore, sustainable energy sources have gained enormous importance. With respect to ethanol fuel production, biomass is gradually replacing grain as the main raw material. In this study, we explored the fermentation of five- and six-carbon sugars, the main biomass degradation products, into alcohol. We conducted mutagenic screening specifically for Candida tropicalis CICC1779 to obtain a strain that effectively used xylose (Candida tropicalis CICC1779-Dyd). By subsequently studying fermentation conditions under different initial liquid volume oxygen transfer coefficients (kLα), and coupling control of the aeration rate and agitation speed under optimal conditions, the optimal dissolved oxygen change curve was obtained. In addition, we constructed metabolic flow charts and equations to obtain a better understanding of the fermentation mechanism and to improve the ethanol yield. In our experiment, the ethanol production of the wild type stain was 17.58 g·L−1 at a kLα of 120. The highest ethanol yield of the mutagenic strains was 24.85 g·L−1. The ethanol yield increased to 26.56 g·L−1 when the dissolved oxygen content was optimized, and the conversion of sugar into alcohol reached 0.447 g·g−1 glucose (the theoretical titer of yeast-metabolized xylose was 0.46 g ethanol/g xylose and the glucose ethanol fermentation titer was 0.51 g ethanol/g glucose). Finally, the detected activity of xylose reductase and xylose dehydrogenase was higher in the mutant strain than in the original, which indicated that the mutant strain (CICC1779-Dyd) could effectively utilize xylose for metabolism.
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影响因子：
8.4
作者：
McKinlay, James B.;Shachar-Hill, Yair;Vieille, Claire
通讯作者：
Vieille, Claire
影响因子：
3
作者：
Guo, Jian;Li, Junxia;Xiao, Dongguang
通讯作者：
Xiao, Dongguang
影响因子：
3.9
作者：
Khare, Sunil K.;Pandey, Ashok;Larroche, Christian
通讯作者：
Larroche, Christian
影响因子：
11.4
作者：
Quintero, Julian A.;Moncada, Jonathan;Cardona, Carlos A.
通讯作者：
Cardona, Carlos A.
影响因子：
4.3
作者：
Riascos, CAM;Gombert, AK;Pinto, JM
通讯作者：
Pinto, JM