Evaluation of the Effects of Pentagastrin, Gastrin and Pancreatic Glucagon On Cell Proliferation In the Rat Gastrointestinal Tract
Evaluation of the Effects of Pentagastrin, Gastrin and Pancreatic Glucagon On Cell Proliferation In the Rat Gastrointestinal Tract
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五肽胃泌素、胃泌素和胰高血糖素对大鼠胃肠道细胞增殖的影响评价
DOI：
10.1111/j.1365-2184.1984.tb00574.x
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发表时间：
1984
影响因子：
8.5
通讯作者：
J. Sharp
中科院分区：
文献类型：
--
作者：
S. Fatemi;G. E. Cullan;J. Sharp
研究了六次注射不同剂量（100-1000 μg/kg体重）的五肽胃泌素和单次注射不同剂量的猪胃泌素（10-40 μg/kg体重）和胰高血糖素（25-100 μg/kg体重）对禁食大鼠肠道细胞增殖的影响。采用的终点包括测量胃体、十二指肠、空肠、回肠和结肠的14 C亮氨酸掺入和胸苷衍生的氚含量。测定了50个单独解剖的腺隐窝的三份样品中每μg组织的碳14和氚含量。根据这些数据和清洗、印迹的肠段的湿重，计算隐窝/μg组织和隐窝/肠段的值。结果表明，生理剂量的五肽胃泌素轻微地降低了胃中的细胞增殖，而胃泌素和胰高血糖素没有影响。在小肠中，五肽胃泌素和胃泌素除了增加十二指肠重量外，没有显著影响。相反，高生理剂量的胰高血糖素增加了整个小肠的DNA和蛋白质合成，但特别是在回肠中。药理剂量的五肽胃泌素和所有剂量的胃泌素似乎都会增加结肠中的细胞增殖，尽管不能排除这是由于刺激前体吸收的可能性。胃泌素也增加结肠重量。胰高血糖素对结肠无影响。这些观察结果与以下假设一致：（i）胃泌素和五肽胃泌素对近端肠的主要作用是作为促分泌素，对细胞增殖的作用可能是次要的，（ii）生理水平的胃泌素和五肽胃泌素不刺激小肠细胞增殖，但胰高血糖素刺激，和（iii）生理水平的胃泌素和药理水平的五肽胃泌素可以刺激结肠中的细胞增殖。
ABSTRACT The effects of six injections of a range of doses (100–1000 μg/kg bodyweight) of pentagrastrin and single injection of a range of doses of porcine gastrin (10–40 μg/kg bodyweight) and pancreatic glucagon (25–100 μg/kg bodyweight) on cell proliferation in the intestine of fasted rats has been investigated. the end‐point employed included the measurement of 14C leucine incorporation and thymidine‐derived tritium content of the body of the stomach, duodenum, jejunum, ileum and colon. the carbon 14 and tritium content per μg of tissue in triplicate samples of fifty individually dissected crypts of glands were determined. From these data and the wet weight of the washed, blotted, intestinal segments, values for crypts/μg tissue and crypts/segment were calculated. the results demonstrated that pentagastrin at physiological doses decreased cell proliferation slightly in stomach, while gastrin and glucagon were without effect. In the small intestine, pentagastrin and gastrin were without significant effect with the exception that they increased the weight of the duodenum. In contrast, a high physiological dose of glucagon increased DNA and protein synthesis throughout the small bowel, but particularly in the ileum. Pharmacological doses of pentagastrin and all doses of gastrin appeared to increase cell proliferation in the colon although the possibility could not be excluded that this was due to stimulation of precursor uptake. Gastrin also increased colonic weight. Glucagon had no effects in the colon. These observations are compatible with the hypothesis that (i) the primary effects of gastrin and pentagastrin on the proximal intestine are as secretogogues and effects on cell proliferation may be secondary, (ii) gastrin and pentagastrin at physiological levels do not stimulate small intestinal cell proliferation, however glucagon does, and (iii) gastrin at physiological levels and pentagastrin at pharmacological levels may stimulate cell proliferation in the colon.
胃泌素在小肠切除术后胃肠适应中的作用。
DOI：
10.1152/ajpgi.1982.243.1.g16
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
Dembinski,AB;Johnson,LR
通讯作者：
Johnson,LR