Effects of manganese on the microstructures of Chenopodium ambrosioides L., A manganese tolerant plant

Effects of manganese on the microstructures of Chenopodium ambrosioides L., A manganese tolerant plant
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锰对耐锰植物土藜微观结构的影响

DOI:
10.1080/15226514.2015.1131233
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发表时间:
2016-04
影响因子:
3.7
通讯作者:
W. Pan
W. Pan
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
C. Wu;J. Lei;W. Hartley;W. Pan

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摘要藜能耐受高浓度的锰,在锰矿尾矿的植被恢复中具有潜在的应用前景。在水培条件下,利用透射电子显微镜-能谱技术研究了不同锰处理(200、1000、10000μ·L−1)对黄花茶叶片细胞微结构的变化及叶片细胞中锰的可能积累。在2 0 0μmoL·L−1浓度下,蛇床子的超微结构清晰可见,未见明显损伤。在1000molmol.L−1浓度下,根、茎、叶细胞完好,细胞器清晰可见,无明显损伤。然而,当锰浓度超过1000molmol.L−1时,根细胞线粒体数量减少,干细胞叶绿体中基粒片层和嗜奥斯粒减少。与对照组相比,1000μ·L−1或10000μ·L−1 mN处理30天后,细胞内出现黑色凝集。在10000μ·L−1浓度下,叶片细胞和细胞间隙中可观察到锰形成针状结构,这可能是茶树对锰的耐受和积累的一种形式。本研究加深了对植物耐锰机制的认识,为锰污染土壤的植被修复提供了新的思路。
ABSTRACT Chenopodium ambrosioides L. can tolerate high concentrations of manganese and has potential for its use in the revegetation of manganese mine tailings. Following a hydroponic investigation, transmission electron microscopy (TEM)-energy disperse spectroscopy (EDS) was used to study microstructure changes and the possible accumulation of Mn in leaf cells of C. ambrosioides in different Mn treatments (200, 1000, 10000 μmol·L−1). At 200 μmol·L−1, the ultrastructure of C. ambrosioides was clearly visible without any obvious damage. At 1000 μmol·L−1, the root, stem and leaf cells remained intact, and the organelles were clearly visible without any obvious damage. However, when the Mn concentration exceeded 1000 μmol·L−1 the number of mitochondria in root cells decreased and the chloroplasts in stem cells showed a decrease in grana lamellae and osmiophilic granules. Compared to controls, treatment with 1000 μmol·L−1 or 10000 μmol·L−1 Mn over 30 days, gave rise to black agglomerations in the cells. At 10000 μmol·L−1, Mn was observed to form acicular structures in leaf cells and intercellular spaces, which may be a form of tolerance and accumulation of Mn in C. ambrosioides. This study has furthered the understanding of Mn tolerance mechanisms in plants, and is potential for the revegetation of Mn-polluted soils.
DOI: --
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