A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat

A carbohydrate-binding protein, FLOURY ENDOSPERM 6 influences the initiation of A- and B-type starch granules in wheat
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FLOURY ENDOSPERM 6 是一种碳水化合物结合蛋白,可影响小麦中 A 型和 B 型淀粉颗粒的形成

DOI:
10.1101/643759
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发表时间:
2019
期刊:
--
影响因子:
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通讯作者:
Chia T
Chia T
中科院分区:
--
文献类型:
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作者:
Chia T

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在此之前,我们确定了山羊草和面包小麦的第4组染色体上的数量性状位点,控制B型淀粉颗粒含量。在这里,我们确定了一个候选基因的精细定位山羊草,并确认其功能小麦TILLING突变体。该基因与水稻和大麦的FLOURY ENDOSPERM 6(FLO 6)基因和拟南芥的PTST 2基因是直向的。在小麦族胚乳中,功能性FLO 6等位基因的基因剂量的减少导致B-颗粒的减少或损失。这是由于在晚粒发育过程中抑制了颗粒的起始,但对A颗粒的合成没有不利影响。然而,功能性FLO 6的完全缺失导致正常A型和B型颗粒的数量减少,并产生大小和形状不同的高度异常颗粒。这种在wheatflo 6三重突变体中观察到的多晶型淀粉与在大麦突变体Franubet中观察到的相似。对Franubet(破碎的Nubet)淀粉的分析表明,突变体A颗粒不是破碎的,而是复合的,这是由于在早期籽粒发育期间质体中颗粒起始的刺激。因此,在小麦族的不同情况下,FLO 6刺激或抑制颗粒起始。
Previously, we identified a quantitative trait locus on the group 4 chromosomes of Aegilops and bread wheat that controls B-type starch-granule content. Here, we identify a candidate gene by fine-mapping in Aegilops and confirm its function using wheat TILLING mutants. This gene is orthologous to theFLOURY ENDOSPERM 6(FLO6) gene of rice and barley and the PTST2 gene of Arabidopsis. In Triticeae endosperm, reduction in the gene dose of functionalFLO6alleles results in reduction, or loss, of B-granules. This is due to repression of granule initiation in late-grain development, but has no deleterious impact on the synthesis of A-granules. The complete absence of functional FLO6, however, results in reduced numbers of normal A-type and B-type granules and the production of highly-abnormal granules that vary in size and shape. This polymorphous starch seen in a wheatflo6triple mutant is similar to that observed in the barley mutant Franubet. Analysis of Franubet (fractured Nubet) starch suggests that the mutant A-granules are not fractured but compound, due to stimulation of granule initiation in plastids during early-grain development. Thus, in different situations in Triticeae, FLO6 either stimulates or represses granule initiation.
DOI: --
发表时间: 1983
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影响因子: --
作者:
田中 正武;河原 太八
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发表时间: 2018-08-17
期刊: SCIENCE
影响因子: 56.9
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发表时间: 2010
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