Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes.
Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes.
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DOI：
10.1038/s41467-023-43004-9
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发表时间：
2023-11-14
影响因子：
16.6
通讯作者：
Edel, Joshua B.
中科院分区：
文献类型：
--
作者：
Ren, Ren;Cai, Shenglin;Fang, Xiaona;Wang, Xiaoyi;Zhang, Zheng;Damiani, Micol;Hudlerova, Charlotte;Rosa, Annachiara;Hope, Joshua;Cook, Nicola J.;Gorelkin, Peter;Erofeev, Alexander;Novak, Pavel;Badhan, Anjna;Crone, Michael;Freemont, Paul;Taylor, Graham P.;Tang, Longhua;Edwards, Christopher;Shevchuk, Andrew;Cherepanov, Peter;Luo, Zhaofeng;Tan, Weihong;Korchev, Yuri;Ivanov, Aleksandar P.;Edel, Joshua B.
我们报道了单分子纳米孔传感与位置编码DNA分子探针相结合的方法，通过化学调节以高灵敏度和选择性同时识别SARS-CoV-2的各种抗原蛋白和多个RNA基因片段。我们发现，这种核糖核酸传感策略可以直接检测未经处理的人类唾液中的Spike(S)和核衣壳(N)蛋白。此外，我们的方法能够使用鼻拭子/喉咙拭子从患者样本中识别RNA片段，从而能够识别病毒变异中的关键突变，如D614G、G446S或Y144del。特别是，它可以在一次测量中检测和区分野生型B.1.1.7(Alpha)、B.1.617.2(Delta)和B.1.1.539(Omicron)的SARS-CoV-2谱系，而不需要核酸测序。分子探针的传感策略很容易适应其他病毒靶点和疾病，并可以根据需要的应用进行扩展。在临床样本中快速区分SARS-CoV-2变种仍然是一个挑战。在这里，作者报道了单分子纳米孔传感与DNA分子探针相结合，同时检测患者样本中SARS-CoV-2变异株的各种抗原和RNA突变。
We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, with chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity. We show that this sensing strategy can directly detect spike (S) and nucleocapsid (N) proteins in unprocessed human saliva. Moreover, our approach enables the identification of RNA fragments from patient samples using nasal/throat swabs, enabling the identification of critical mutations such as D614G, G446S, or Y144del among viral variants. In particular, it can detect and discriminate between SARS-CoV-2 lineages of wild-type B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.1.539 (Omicron) within a single measurement without the need for nucleic acid sequencing. The sensing strategy of the molecular probes is easily adaptable to other viral targets and diseases and can be expanded depending on the application required. Fast discrimination of SARS-CoV-2 variants in clinical samples remains a challenge. Here, authors report on single molecule nanopore sensing combined with DNA molecular probes to simultaneously detect various antigens and RNA mutations of SARS-CoV-2 variants in patient samples.
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影响因子：
16.6
作者：
Cai S;Pataillot-Meakin T;Shibakawa A;Ren R;Bevan CL;Ladame S;Ivanov AP;Edel JB
通讯作者：
Edel JB
影响因子：
16.6
作者：
Freedman KJ;Otto LM;Ivanov AP;Barik A;Oh SH;Edel JB
通讯作者：
Edel JB
影响因子：
1.7
作者：
Ochola L;Ogongo P;Mungai S;Gitaka J;Suliman S
通讯作者：
Suliman S
影响因子：
9.4
作者：
Lee RA;Herigon JC;Benedetti A;Pollock NR;Denkinger CM
通讯作者：
Denkinger CM
DOI：
10.1056/nejmoa2119451
发表时间：
2022-04-21
期刊：
The New England journal of medicine
影响因子：
--
作者：
Andrews N;Stowe J;Kirsebom F;Toffa S;Rickeard T;Gallagher E;Gower C;Kall M;Groves N;O'Connell AM;Simons D;Blomquist PB;Zaidi A;Nash S;Iwani Binti Abdul Aziz N;Thelwall S;Dabrera G;Myers R;Amirthalingam G;Gharbia S;Barrett JC;Elson R;Ladhani SN;Ferguson N;Zambon M;Campbell CNJ;Brown K;Hopkins S;Chand M;Ramsay M;Lopez Bernal J
通讯作者：
Lopez Bernal J