Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum.
Single-molecule amplification-free multiplexed detection of circulating microRNA cancer biomarkers from serum.
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DOI：
10.1038/s41467-021-23497-y
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发表时间：
2021-06-10
影响因子：
16.6
通讯作者：
Edel JB
中科院分区：
文献类型：
--
作者：
Cai S;Pataillot-Meakin T;Shibakawa A;Ren R;Bevan CL;Ladame S;Ivanov AP;Edel JB
微小RNA（miRNAs）在转录后基因表达中起重要作用，并且还在体液如血液中自由循环。失调的miRNA特征与包括癌症在内的许多疾病相关，并且来自液体活检的miRNA谱分析为癌症诊断、预后和监测提供了有希望的策略。在这里，我们开发了大小编码的分子探针，可用于miRNA的同时电光纳米孔传感，允许直接在未加工的人血清中进行超灵敏，序列特异性和多重检测，样品体积小至0.1 μl。我们表明，这种方法允许飞摩尔灵敏度和单碱基错配选择性。我们证明了同时监测活动性疾病和缓解期前列腺癌患者的miRNA（miR-141- 3 p和miR-375- 3 p）的能力。这项技术可以为下一代癌症微创诊断和伴随诊断测试铺平道路。来自患者血液的miRNA谱可用于癌症诊断。在此，作者提出了一种电光纳米孔传感平台，该平台允许直接在人血清中灵敏和特异地检测miRNA，并适用于监测不同阶段的前列腺癌中的miR-141- 3 p和miR-375- 3 p。
MicroRNAs (miRNAs) play essential roles in post-transcriptional gene expression and are also found freely circulating in bodily fluids such as blood. Dysregulated miRNA signatures have been associated with many diseases including cancer, and miRNA profiling from liquid biopsies offers a promising strategy for cancer diagnosis, prognosis and monitoring. Here, we develop size-encoded molecular probes that can be used for simultaneous electro-optical nanopore sensing of miRNAs, allowing for ultrasensitive, sequence-specific and multiplexed detection directly in unprocessed human serum, in sample volumes as small as 0.1 μl. We show that this approach allows for femtomolar sensitivity and single-base mismatch selectivity. We demonstrate the ability to simultaneously monitor miRNAs (miR-141-3p and miR-375-3p) from prostate cancer patients with active disease and in remission. This technology can pave the way for next generation of minimally invasive diagnostic and companion diagnostic tests for cancer. miRNA profiling from patient blood can be used for cancer diagnosis. Here the authors present an electro-optical nanopore sensing platform which allows sensitive and specific miRNA detection directly in human serum and apply to monitoring of miR-141-3p and miR-375-3p in different stage of prostate cancer.
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影响因子：
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作者：
Chau, Chalmers C.;Radford, Sheena E.;Actis, Paolo
通讯作者：
Actis, Paolo
影响因子：
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Omranipour, Ramesh
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Guegler KJ
影响因子：
10.8
作者：
Cadinu P;Paulose Nadappuram B;Lee DJ;Sze JYY;Campolo G;Zhang Y;Shevchuk A;Ladame S;Albrecht T;Korchev Y;Ivanov AP;Edel JB
通讯作者：
Edel JB