Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells.
Histone deacetylase inhibitors up-regulate LL-37 expression independent of toll-like receptor mediated signalling in airway epithelial cells.
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DOI：
10.1186/1476-9255-10-15
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发表时间：
2013-04-11
期刊：
影响因子：
--
通讯作者：
van Drunen CM
中科院分区：
文献类型：
--
作者：
Liu Q;Liu J;Roschmann KIL;van Egmond D;Golebski K;Fokkens WJ;Wang D;van Drunen CM
HDAC inhibitors have been proposed as anticancer agents. However, their roles in innate genes expression remain not well known. Cathelicidin LL-37 is one of the few human bactericidal peptides, but the regulation of histone acetylation on LL-37 expression in airway epithelium remains largely unknown. Therefore, we investigated the effects of two non-selective HDACi, trichostatin A (TSA) and sodium butyrate (SB), on the expression of the cathelicidin LL-37 in human airway epithelial cells. LL37 in human NCI-H292 airway epithelial cells and the primary cultures of normal nasal epithelial cells(PNEC) in response to HDAC inhibitors with or without poly (I:C) stimulation was assessed using real-time PCR and western blot. In parallel, IL-6 expression was evaluated by ELISA. Our results showed that HDAC inhibitors up-regulated LL-37 gene expression independent of poly (I:C) stimulation in PNEC as well as in NCI-H292 cells. HDAC inhibitors increased LL37 protein expression in NCI-H292 cells but not in PNEC. In addition, HDAC inhibitors significantly inhibited poly (I:C)-induced IL-6 production in both of the epithelial cells. In conclusion, HDAC inhibitors directly up-regulated LL-37 gene expression in human airway epithelial cells.
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影响因子：
6.2
作者：
Sub, Hyeon-Sook;Choi, Shinyeop;Khattar, Pallavi;Choi, Namjong;Lee, Sunhee C.
通讯作者：
Lee, Sunhee C.
影响因子：
8
作者：
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影响因子：
9.2
作者：
Imhof, A;Wolffe, AP
通讯作者：
Wolffe, AP
影响因子：
3.4
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Redfern, Rachel L.;Reins, Rose Y.;McDermott, Alison M.
通讯作者：
McDermott, Alison M.
影响因子：
120.1
作者：
Wang, Liqing;de Zoeten, Edwin F.;Greene, Mark I.;Hancock, Wayne W.
通讯作者：
Hancock, Wayne W.