Identification of long-grain chromosome segment substitution line Z744 and QTL analysis for agronomic traits in rice
Identification of long-grain chromosome segment substitution line Z744 and QTL analysis for agronomic traits in rice
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水稻长粒染色体片段替代系Z744鉴定及农艺性状QTL分析
DOI：
10.1016/s2095-3119(19)62751-6
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发表时间：
2020-05
影响因子：
4.8
通讯作者：
ZHAO Fang-ming
中科院分区：
文献类型：
--
作者：
MA Fu-ying;DU Jie;WANG Da-chuan;WANG Hui;ZHAO Bing-bing;HE Guang-hua;YANG Zheng-lin;ZHANG Ting;WU Ren-hong;ZHAO Fang-ming
粒长影响水稻的外观、品质和产量。鉴定出了一个以日本晴为受体亲本、西恢18为供体亲本的水稻长粒染色体片段代换系Z744。Z744共包含6个代换片段，分布在第1、2、6、7和12号染色体上，平均代换长度为2.72 Mb。Z744的粒长、长宽比和千粒重显著高于日本晴。Z744的株高、穗数和结实率显著低于日本晴，但仍分别达到78.7厘米、每株13.5个和86.49%。此外，在日本晴与Z744杂交构建的次级F2群体中鉴定出8个不同性状的数量性状基因座（QTL）。Z744的粒重分别受2个增效QTL（qGWT1和qGWT7）和2个减效QTL（qGWT2和qGWT6）控制。Z744粒重增加主要是由于粒长增加。检测到2个QTL，即qGL1和qGL7 - 3，它们分别解释25.54%和15.58%的表型变异。卡方检验表明，长粒数与短粒数符合3:1的分离比例，这表明长粒对短粒为显性，且Z744主要受主效基因qGL1控制。这些结果为qGL1的进一步精细定位以及将其他QTL进一步解析为单片段代换系提供了良好基础。
Length of grain affects the appearance, quality, and yield of rice. A rice long-grain chromosome segment substitution line .Z744, with Nipponbare as the recipient parent and Xihui 18 as the donor parent, was identified. Z744 contains a total of six .substitution segments distributed on chromosomes (Chrs.) 1, 2, 6, 7, and 12, with an average substitution length of 2.72 Mb. .The grain length, ratio of length to width, and 1000-grain weight of Z744 were significantly higher than those in Nipponbare. .The plant height, panicle number, and seed-set ratio in Z744 were significantly lower than those in Nipponbare, but they .were still 78.7 cm, 13.5 per plant, and 86.49%, respectively. Furthermore, eight QTLs of different traits were identified in the .secondary F2. population, constructed by Nipponbare and Z744 hybridization. The grain weight of Z744 was controlled by .two synergistic QTLs (qGWT1 and qGWT7) and two subtractive QTLs (qGWT2 and qGWT6), respectively. The increase in .the grain weight of Z744 was caused mainly by the increase in grain length. Two QTLs were detected, qGL1 and qGL7-3, .which accounted for 25.54 and 15.58% of phenotypic variation, respectively. A Chi-square test showed that the long-grain .number and the short-grain number were in accordance with the 3:1 separation ratio, which indicates that the long grain is .dominant over the short-grain and Z744 was controlled mainly by the principal effect qGL1. These results offered a good .basis for further fine mapping of qGL1 and further dissection of other QTLs into single-segment substitution lines.
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