Expressed sequence tag analysis in Cycas, the most primitive living seed plant.
Expressed sequence tag analysis in Cycas, the most primitive living seed plant.
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DOI：
10.1186/gb-2003-4-12-r78
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发表时间：
2003
期刊：
影响因子：
12.3
通讯作者：
Coruzzi GM
中科院分区：
文献类型：
--
作者：
Brenner ED;Stevenson DW;McCombie RW;Katari MS;Rudd SA;Mayer KF;Palenchar PM;Runko SJ;Twigg RW;Dai G;Martienssen RA;Benfey PN;Coruzzi GM
苏铁ESTs分析揭示了保守的和潜在的新基因。苏铁中谷氨酸受体激动剂和谷氨酸受体样基因的存在，支持了这样一种假设，即这些具有神经活性的植物产物不仅是食草动物的威慑物，而且可能在植物信号传导中起作用。苏铁是古老的种子植物（活化石），起源于古生代。苏铁有时被认为是一个“缺失的环节”，因为它们表现出介于维管非种子植物和更多衍生种子植物之间的特征。苏铁也被认为是“关岛痴呆”的来源，可能是由于产生S(+)- β -甲基- α， β -二氨基丙酸（BMAA），这是一种动物谷氨酸受体的激动剂。从苏铁中共获得4200个表达序列标签（est），聚类为2458个contigs，其中1764个与其他植物基因具有低强度BLAST相似性。在这些与植物基因相似的苏铁序列中，1718个与被子植物“匹配”，1310个与裸子植物“匹配”，734个与低等（非种子）植物“匹配”。在低等植物和裸子植物中发现了46个只匹配基因的序列。从37/46个contigs克隆中获得完整序列后，仍有14个只匹配裸子植物。在高等植物中常见的苏铁组群中，发现了与现代开花植物发育和信号传递相关的ESTs。我们纯化了谷氨酸受体（GLR）样基因的苏铁EST，以及可能参与GLR激动剂BMAA合成的EST。苏铁ESTs分析揭示了保守的和潜在的新基因。此外，苏铁中谷氨酸受体激动剂和谷氨酸受体样基因的存在，支持了这样一种假设，即这些神经活性植物产物不仅是食草动物的威慑物，而且可能在植物信号传导中起作用。
Analysis of cycad ESTs has uncovered conserved and potentially novel genes. The presence of a glutamate receptor agonist, as well as a glutamate receptor-like gene in cycads, supports the hypothesis that such neuroactive plant products are not merely herbivore deterrents but may also serve a role in plant signaling. Cycads are ancient seed plants (living fossils) with origins in the Paleozoic. Cycads are sometimes considered a 'missing link' as they exhibit characteristics intermediate between vascular non-seed plants and the more derived seed plants. Cycads have also been implicated as the source of 'Guam's dementia', possibly due to the production of S(+)-beta-methyl-alpha, beta-diaminopropionic acid (BMAA), which is an agonist of animal glutamate receptors. A total of 4,200 expressed sequence tags (ESTs) were created from Cycas rumphii and clustered into 2,458 contigs, of which 1,764 had low-stringency BLAST similarity to other plant genes. Among those cycad contigs with similarity to plant genes, 1,718 cycad 'hits' are to angiosperms, 1,310 match genes in gymnosperms and 734 match lower (non-seed) plants. Forty-six contigs were found that matched only genes in lower plants and gymnosperms. Upon obtaining the complete sequence from the clones of 37/46 contigs, 14 still matched only gymnosperms. Among those cycad contigs common to higher plants, ESTs were discovered that correspond to those involved in development and signaling in present-day flowering plants. We purified a cycad EST for a glutamate receptor (GLR)-like gene, as well as ESTs potentially involved in the synthesis of the GLR agonist BMAA. Analysis of cycad ESTs has uncovered conserved and potentially novel genes. Furthermore, the presence of a glutamate receptor agonist, as well as a glutamate receptor-like gene in cycads, supports the hypothesis that such neuroactive plant products are not merely herbivore deterrents but may also serve a role in plant signaling.
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影响因子：
6.4
作者：
BOGDANOV.M
通讯作者：
BOGDANOV.M
影响因子：
9.2
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL
影响因子：
64.5
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL
DOI：
10.1073/pnas.1132171100
发表时间：
2003-06-10
影响因子：
11.1
作者：
Kirst, M;Johnson, AF;Sederoff, R
通讯作者：
Sederoff, R
影响因子：
7.4
作者：
Brenner, ED;Martinez-Barboza, N;Coruzzi, GM
通讯作者：
Coruzzi, GM