Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging.
Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging.
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DOI：
10.1038/s41596-021-00530-4
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发表时间：
2021-06
期刊：
影响因子：
14.8
通讯作者：
Landry, Markita P.
中科院分区：
文献类型：
--
作者：
Yang, Sarah J.;Del Bonis-O'Donnell, Jackson Travis;Beyene, Abraham G.;Landry, Markita P.
神经突触的多巴胺神经调节是涉及许多关键脑功能和疾病的过程。开发可视化这种动态神经化学过程的协议对于理解多巴胺如何调节大脑功能至关重要。我们开发了一种非基因编码的近红外（nIR）儿茶酚胺纳米传感器（nIRCat），能够识别背侧纹状体脑片中的~2 μm多巴胺释放热点。nIRCat易于通过超声处理单壁碳纳米管与DNA寡核苷酸合成，可易于引入遗传上易处理和难处理的生物体中，并且与许多多巴胺受体激动剂和拮抗剂相容。在这里，我们描述的合成，表征和实施nIRCat在急性小鼠脑切片。我们演示了如何nIRCat可以用来成像电或光遗传刺激多巴胺释放，以及如何利用这些程序来研究多巴胺受体药理学的影响。此外，我们提供建议，建设或适应宽视场显微镜，以兼容nIRCat nIR荧光成像。我们讨论了分析近红外视频数据，以确定多巴胺释放热点和量化其动力学的策略。该协议可以适应和实施成像其他神经调质通过使用这一类的探针，并可以用于广泛的物种没有遗传操作。nIRCat的合成和表征方案需要约5小时，并且通过使用nIRCats的活脑切片的制备和荧光成像需要约6小时。
Dopamine neuromodulation of neural synapses is a process implicated in a number of critical brain functions and diseases. Development of protocols to visualize this dynamic neurochemical process is essential to understanding how dopamine modulates brain function. We have developed a non-genetically encoded, near-IR (nIR) catecholamine nanosensor (nIRCat) capable of identifying ~2-μm dopamine release hotspots in dorsal striatal brain slices. nIRCat is readily synthesized through sonication of single walled carbon nanotubes with DNA oligos, can be readily introduced into both genetically tractable and intractable organisms and is compatible with a number of dopamine receptor agonists and antagonists. Here we describe the synthesis, characterization and implementation of nIRCat in acute mouse brain slices. We demonstrate how nIRCat can be used to image electrically or optogenetically stimulated dopamine release, and how these procedures can be leveraged to study the effects of dopamine receptor pharmacology. In addition, we provide suggestions for building or adapting wide-field microscopy to be compatible with nIRCat nIR fluorescence imaging. We discuss strategies for analyzing nIR video data to identify dopamine release hotspots and quantify their kinetics. This protocol can be adapted and implemented for imaging other neuromodulators by using probes of this class and can be used in a broad range of species without genetic manipulation. The synthesis and characterization protocols for nIRCat take ~5 h, and the preparation and fluorescence imaging of live brain slices by using nIRCats require ~6 h.
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影响因子：
10.8
作者：
Beyene AG;Alizadehmojarad AA;Dorlhiac G;Goh N;Streets AM;Král P;Vuković L;Landry MP
通讯作者：
Landry MP
神经元型特异性信号，用于腹侧对段区域的奖励和惩罚。
影响因子：
64.8
作者：
Cohen, Jeremiah Y.;Haesler, Sebastian;Vong, Linh;Lowell, Bradford B.;Uchida, Naoshige
通讯作者：
Uchida, Naoshige
DOI：
10.1126/science.aat4422
发表时间：
2018-06-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Patriarchi T;Cho JR;Merten K;Howe MW;Marley A;Xiong WH;Folk RW;Broussard GJ;Liang R;Jang MJ;Zhong H;Dombeck D;von Zastrow M;Nimmerjahn A;Gradinaru V;Williams JT;Tian L
通讯作者：
Tian L
影响因子：
56.9
作者：
O'Connell, MJ;Bachilo, SM;Smalley, RE
通讯作者：
Smalley, RE
影响因子：
4.3
作者：
Romano SA;Pérez-Schuster V;Jouary A;Boulanger-Weill J;Candeo A;Pietri T;Sumbre G
通讯作者：
Sumbre G