Addressing the role of microRNAs in reprogramming leaf growth during drought stress in Brachypodium distachyon.
Addressing the role of microRNAs in reprogramming leaf growth during drought stress in Brachypodium distachyon.
复制标题
DOI：
10.1093/mp/sss160
复制
发表时间：
2013-03
期刊：
影响因子：
27.5
通讯作者：
Mica E
中科院分区：
文献类型：
--
作者：
Bertolini E;Verelst W;Horner DS;Gianfranceschi L;Piccolo V;Inzé D;Pè ME;Mica E
我们研究了已知和新发现的miRNAs在短柄青霉干旱反应和叶片发育中的作用。差异表达分析和miRNA靶标预测表明，在正常和应激条件下，调控网络控制细胞分裂和扩张。植物对干旱的反应由复杂的遗传和表观遗传网络调节，导致植物生长的快速重新编程。MiRNAs已被广泛认为是调节生长和发育的关键角色。研究了miRNAs在耐旱单子叶模式植物短柄短臂幼叶干旱反应中的作用。采用体内干旱分析，表明导致叶大小急剧减小，主要是由于细胞扩张减少，从增殖和扩张的叶细胞中产生了小的RNA文库。使用内部生物信息学管道分析了下一代测序数据，从而识别了66个带注释的miRNA基因和122个新的高置信度预测，极大地扩大了已知Brachypodium miRNAs的数量。此外，我们还鉴定了四个TAS3基因座和大量的siRNA产生基因座，这些基因座显示出可能代表年轻miRNA基因的特征。大多数miRNAs的表达水平都很高，这与它们参与叶片早期发育和细胞鉴定是一致的。增殖和膨大的叶片细胞对干旱处理的反应不同，差异表达分析表明，在正常和胁迫条件下，miRNA调控网络控制细胞分裂，并表明干旱触发了叶片生长的遗传重新编程，miRNAs深度参与其中。
We investigated the role of known and newly discovered miRNAs in drought response and leaf development in Brachypodium distachyon. Differential expression analyses and miRNA-target predictions suggest evidence for regulatory networks controlling cell division and expansion in normal and stressed conditions. Plant responses to drought are regulated by complex genetic and epigenetic networks leading to rapid reprogramming of plant growth. miRNAs have been widely indicated as key players in the regulation of growth and development. The role of miRNAs in drought response was investigated in young leaves of Brachypodium distachyon, a drought-tolerant monocot model species. Adopting an in vivo drought assay, shown to cause a dramatic reduction in leaf size, mostly due to reduced cell expansion, small RNA libraries were produced from proliferating and expanding leaf cells. Next-generation sequencing data were analyzed using an in-house bioinformatics pipeline allowing the identification of 66 annotated miRNA genes and 122 new high confidence predictions greatly expanding the number of known Brachypodium miRNAs. In addition, we identified four TAS3 loci and a large number of siRNA-producing loci that show characteristics suggesting that they may represent young miRNA genes. Most miRNAs showed a high expression level, consistent with their involvement in early leaf development and cell identity. Proliferating and expanding leaf cells respond differently to drought treatment and differential expression analyses suggest novel evidence for an miRNA regulatory network controlling cell division in both normal and stressed conditions and demonstrate that drought triggers a genetic reprogramming of leaf growth in which miRNAs are deeply involved.
登录
查看更多内容
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
7.4
作者：
Alonso-Peral, Maria M.;Li, Junyan;Millar, Anthony A.
通讯作者：
Millar, Anthony A.
影响因子：
7.4
作者：
Aung, Kyaw;Lin, Shu-I;Chiou, Tzyy-Jen
通讯作者：
Chiou, Tzyy-Jen
影响因子：
2.7
作者：
Donnelly, PM;Bonetta, D;Dengler, NG
通讯作者：
Dengler, NG