Increased adipose tissue hypoxia and capacity for angiogenesis and inflammation in young diet-sensitive C57 mice compared with diet-resistant FVB mice.
Increased adipose tissue hypoxia and capacity for angiogenesis and inflammation in young diet-sensitive C57 mice compared with diet-resistant FVB mice.
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DOI：
10.1038/ijo.2012.141
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发表时间：
2013-06
影响因子：
4.9
通讯作者：
Seeley, R. J.
中科院分区：
文献类型：
--
作者：
Kim, D-H;Gutierrez-Aguilar, R.;Kim, H-J;Woods, S. C.;Seeley, R. J.
高脂肪饮食导致体重增加。然而，这是不统一的，确定使一些动物或个体更容易受到这种饮食诱导的体重增加的因素是一个关键的研究问题。与体重增加相关的白色脂肪组织（WAT）的扩张需要高血管生成率来支持扩张的组织块。我们假设，饮食诱导的肥胖（DIO）小鼠有更大的能力，WAT血管生成和重塑比饮食抵抗（DR）小鼠在年轻的时候，年龄或饮食诱导的肥胖之前。我们通过NMR测量体重和身体成分。我们通过RT-qPCR和PCR阵列比较了脂质代谢、血管生成和炎症相关基因的表达。分析WAT形态及脂肪细胞大小分布。采用免疫组化方法检测幼鼠WAT的缺氧程度和血管密度。C57 B1/6小鼠是在低脂肪饮食或高脂肪饮食（HFD）8周后的DIO和FVB/N小鼠DR。然而，C57 Bl/6小鼠在9周龄时具有较低的体重、较低的肥胖、较小的脂肪细胞以及AT中的瘦素和脂肪生成基因表达低于普通饲料喂养的FVB/N小鼠。尽管具有较小的脂肪细胞，但年轻C57 Bl/6小鼠的WAT中的缺氧水平和促血管生成基因的表达高于年轻FVB/N小鼠。此外，与巨噬细胞及其募集相关的基因以及促炎细胞因子的表达在年轻C57 Bl/6小鼠的WAT中显著高于年轻FVB/N小鼠。这些数据表明，与FVB/N小鼠相比，C57 B1/6小鼠在生长早期的WAT重塑的可能性更高，我们假设这可能有助于C57 B1/6小鼠对DIO的易感性增加。
High-fat diets result in increased body weight. However, this is not uniform and determining the factors that make some animals or individual more susceptible to this diet-induced weight gain is a critical research question. The expansion of white adipose tissue (WAT) associated with weight gain requires high rates of angiogenesis to support the expanding tissue mass. We hypothesized that diet-induced obese (DIO) mice have a greater capacity for WAT angiogenesis and remodeling than diet-resistant (DR) mice at a young age, prior to age or diet-induced obesity. We measured body weight and body composition by NMR. We compared the expression of genes related to lipid metabolism, angiogenesis and inflammation by RT-qPCR and PCR arrays. WAT morphology and distribution of adipocyte size were analyzed. The level of hypoxia and vascular density was assessed by immunohistochemistry in WAT of young mice. C57Bl/6 mice were DIO and FVB/N mice DR after 8 weeks on a low fat diet or high fat diet (HFD). However, C57Bl/6 mice had lower body weight, lower adiposity, smaller adipocytes and decreased leptin and lipogenic genes expression in AT than FVB/N mice at 9 weeks of age on a chow diet. Despite having smaller adipocytes, the level of hypoxia and the expression of pro-angiogenesis genes were higher in WAT of young C57Bl/6 mice than young FVB/N mice. In addition, expression of genes related to macrophages and their recruitment, and to proinflammatory cytokines, was significantly higher in WAT of young C57Bl/6 mice than young FVB/N mice. These data suggest that the potential for WAT remodeling in early period of growth is higher in C57Bl/6 mice as compared to FVB/N mice and we hypothesize that it may contribute to the increased susceptibility to DIO of C57Bl/6 mice.
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影响因子：
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作者：
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DOI：
10.1152/ajpendo.00061.2008
发表时间：
2008-11-01
影响因子：
5.1
作者：
Kim, Dong-Hoon;Sandoval, Darleen;Seeley, Randy J.
通讯作者：
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