Sphingolipid-enriched Membrane Domains from Rat Cerebellar Granule Cells Differentiated in Culture
Sphingolipid-enriched Membrane Domains from Rat Cerebellar Granule Cells Differentiated in Culture
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培养分化的大鼠小脑颗粒细胞富含鞘脂的膜结构域
DOI：
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发表时间：
2000
影响因子：
4.8
通讯作者：
S. Sonnino
中科院分区：
文献类型：
--
作者：
A. Prinetti;V. Chigorno;G. Tettamanti;S. Sonnino
富含鞘脂的膜结构域以特定的蛋白质和脂质组成为特征，已在多种细胞中检测到。然而，关于神经元细胞中这些域的可用数据有限。我们分析了由培养分化的原代大鼠小脑颗粒细胞制备的富含鞘脂的膜组分的脂质和蛋白质组成。虽然该级分的蛋白质含量仅占细胞总蛋白的1.4%，但60%的神经节苷脂、67%的鞘磷脂、50%的神经酰胺和40%的胆固醇都位于该级分中。富含鞘脂的结构域部分的蛋白质模式与细胞匀浆相关的蛋白质模式显着不同。该级分含有 25% 的酪氨酸磷酸化蛋白质，并富含两种表观分子量分别为 135 和 15 kDa 的蛋白质。 12% 的细胞甘油磷脂位于该级分中，其中磷脂酰胆碱的富集度最高。小脑颗粒细胞中蛋白质、甘油磷脂、胆固醇、鞘磷脂、神经酰胺和神经节苷脂之间的摩尔比在细胞匀浆中为1.6:41.6:6.1:1.3:0.3:1，在富含鞘脂的膜部分中为0.04:8.3:4.0:1.4:0.2:1。这些数据表明，选定的蛋白质在培养的神经元膜的特定区域中与鞘脂分离。
Sphingolipid-enriched membrane domains, characterized by a particular protein and lipid composition, have been detected in a variety of cells. However, limited data are available concerning these domains in neuronal cells. We analyzed the lipid and protein composition of a sphingolipid-enriched membrane fraction prepared from primary rat cerebellar granule cells differentiated in culture. Although the protein content of this fraction was only 1.4% of total cellular protein, 60% of the gangliosides, 67% of the sphingomyelin, 50% of the ceramide, and 40% of the cholesterol were located in this fraction. The protein pattern of the sphingolipid-enriched domain fraction was dramatically different from that associated with the cell homogenate. This fraction contained 25% of the tyrosine-phosphorylated proteins and was enriched in two proteins with apparent molecular masses of 135 and 15 kDa. 12% of cellular glycerophospholipids were located in the fraction, with phosphatidylcholine having the highest enrichment. The molar ratio between proteins, glycerophospholipids, cholesterol, sphingomyelin,ceramide and gangliosides in cerebellar granule cells was 1.6:41.6:6.1:1.3:0.3:1 in the cell homogenate and 0.04:8.3:4.0:1.4:0.2:1 in the sphingolipid-enriched membrane fraction. These data indicate that selected proteins segregate with sphingolipids in specialized domains in the membrane of cultured neurons.
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DOI：
10.1073/pnas.91.24.11728
发表时间：
1994-11-22
影响因子：
11.1
作者：
CHUN, MY;LIYANAGE, UK;LODISH, HF
通讯作者：
LODISH, HF
DOI：
10.1073/pnas.92.22.10104
发表时间：
1995-10-24
影响因子：
11.1
作者：
SMART, EJ;YING, YS;ANDERSON, RGW
通讯作者：
ANDERSON, RGW
DOI：
10.1073/pnas.91.25.12130
发表时间：
1994-12-06
影响因子：
11.1
作者：
SCHROEDER, R;LONDON, E;BROWN, D
通讯作者：
BROWN, D
磷脂酰胆碱双层中鞘糖脂的组织：使用抗体分子和 Fab 片段作为形态标记。
影响因子：
2.9
作者：
Rock,P;Allietta,M;YoungJr,WW;Thompson,TE;Tillack,TW
通讯作者：
Tillack,TW