LoCKAmp: lab-on-PCB technology for <3 minute virus genetic detection.
LoCKAmp: lab-on-PCB technology for <3 minute virus genetic detection.
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DOI：
10.1039/d3lc00441d
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发表时间：
2023-10-10
期刊：
影响因子：
6.1
通讯作者：
中科院分区：
文献类型：
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作者：
最近的COVID-19爆发凸显了对适合现场实际部署的芯片实验室诊断技术的需求。多步分析微系统集成和可升级的标准化制造技术的现有瓶颈在疫情期间推迟了芯片实验室解决方案的大规模部署，整个全球诊断测试短缺。这项研究提出了一种技术，有可能通过重新部署和重新利用无处不在的印刷电路板（PCB）技术和制造基础设施来解决这些问题。我们展示了第一个商业化生产的，可重复使用的PCB实验室设备，用于SARS-CoV-2的环介导等温扩增（LAMP）基因检测。该系统集成了大规模生产的连续流PCB芯片和超低成本荧光检测电路，使其成为唯一一个具有现成光学检测组件的连续流μLAMP平台。废水样本中的SARS-CoV-2 RNA扩增速度超快，在2分钟分析内就被证明，浓度低至17 gc μL−1。我们通过检测20个人鼻咽拭子样本中的SARS-CoV-2进一步证明了我们的设备操作，而不需要任何RNA提取或纯化。这使得所提出的基于扩增的核酸扩增的诊断测试成为报道的最快的SARS-CoV-2基因检测平台，在实际实施中适合于现场部署。该技术可以很容易地扩展到检测替代病原体或遗传靶标，用于非常广泛的应用和基质。目前，LoCKeTM lab-on-PCB芯片在符合ISO标准的商业PCB工厂中大规模生产，小规模生产成本为每个芯片2.50英镑。因此，通过这项工作，我们展示了一个高技术准备水平的基于芯片实验室的基因检测系统，成功地对标准分析技术进行了基准测试，用于废水和鼻咽拭子SARS-CoV-2检测。LoCKELL：集成LAMP基因扩增设备，具有可靠的检测和超快的样品到答案时间。
The recent COVID-19 outbreak highlighted the need for lab-on-chip diagnostic technology fit for real-life deployment in the field. Existing bottlenecks in multistep analytical microsystem integration and upscalable, standardized fabrication techniques delayed the large-scale deployment of lab-on-chip solutions during the outbreak, throughout a global diagnostic test shortage. This study presents a technology that has the potential to address these issues by redeploying and repurposing the ubiquitous printed circuit board (PCB) technology and manufacturing infrastructure. We demonstrate the first commercially manufactured, miniaturised lab-on-PCB device for loop-mediated isothermal amplification (LAMP) genetic detection of SARS-CoV-2. The system incorporates a mass-manufactured, continuous-flow PCB chip with ultra-low cost fluorescent detection circuitry, rendering it the only continuous-flow μLAMP platform with off-the-shelf optical detection components. Ultrafast, SARS-CoV-2 RNA amplification in wastewater samples was demonstrated within 2 min analysis, at concentrations as low as 17 gc μL−1. We further demonstrate our device operation by detecting SARS-CoV-2 in 20 human nasopharyngeal swab samples, without the need for any RNA extraction or purification. This renders the presented miniaturised nucleic-acid amplification-based diagnostic test the fastest reported SARS-CoV-2 genetic detection platform, in a practical implementation suitable for deployment in the field. This technology can be readily extended to the detection of alternative pathogens or genetic targets for a very broad range of applications and matrices. LoCKAmp lab-on-PCB chips are currently mass-manufactured in a commercial, ISO-compliant PCB factory, at a small-scale production cost of £2.50 per chip. Thus, with this work, we demonstrate a high technology-readiness-level lab-on-chip-based genetic detection system, successfully benchmarked against standard analytical techniques both for wastewater and nasopharyngeal swab SARS-CoV-2 detection. LoCKAmp: integrated LAMP genetic amplification device demonstrating reliable detection and ultrafast sample-to-answer time.
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DOI：
10.1073/pnas.2014739117
发表时间：
2020-09-15
影响因子：
11.1
作者：
Ganguli A;Mostafa A;Berger J;Aydin MY;Sun F;Ramirez SAS;Valera E;Cunningham BT;King WP;Bashir R
通讯作者：
Bashir R
影响因子：
12.6
作者：
Jolly, Pawan;Rainbow, Joshua;Moschou, Despina
通讯作者：
Moschou, Despina
影响因子：
7.4
作者：
Marshall, Lewis A.;Wu, Liang Li;Santiago, Juan G.
通讯作者：
Santiago, Juan G.
DOI：
10.3390/diagnostics11030438
发表时间：
2021-03-04
期刊：
Diagnostics (Basel, Switzerland)
影响因子：
--
作者：
García-Bernalt Diego J;Fernández-Soto P;Domínguez-Gil M;Belhassen-García M;Bellido JLM;Muro A
通讯作者：
Muro A
SARS-CoV-2 感染的诊断。
影响因子：
41.2
作者：
Kevadiya BD;Machhi J;Herskovitz J;Oleynikov MD;Blomberg WR;Bajwa N;Soni D;Das S;Hasan M;Patel M;Senan AM;Gorantla S;McMillan J;Edagwa B;Eisenberg R;Gurumurthy CB;Reid SPM;Punyadeera C;Chang L;Gendelman HE
通讯作者：
Gendelman HE