Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies.
Bacterial and fungal characterization of pancreatic adenocarcinoma from Endoscopic Ultrasound-guided biopsies.
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DOI：
10.3389/fimmu.2023.1268376
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发表时间：
2023
影响因子：
7.3
通讯作者：
Mcallister, Florencia
中科院分区：
文献类型：
--
作者：
Wright, Robin D.;Bartelli, Thais F.;Baydogan, Seyda;White, James Robert;Kim, Michael P.;Bhutani, Manoop S.;Mcallister, Florencia
关键词：
肿瘤微生物组 (TM) 与胰腺癌的预后有关。特定的微生物可以赋予肿瘤对治疗的抵抗力。诊断时对 TM 的早期了解对于基于微生物组成的精准治疗具有临床意义。然而，在手术切除之前很难定义 TM。在这项试点可行性研究中，患者接受了胰腺癌内镜超声细针抽吸 (EUS-FNA) 活检。使用 16S rRNA 和内转录间隔区 (ITS) 测序对这些样本进行分析，以表征肿瘤细菌和真菌。经过计算机去污并与非匹配肿瘤进行比较后，我们能够表征活检中的 TM，这与手术标本中的 TM 相当。 EUS-FNA 活检可能是一种在手术切除前表征胰腺 TM 的可行方法，并采用适当的净化策略和改进匹配对照。
The tumor microbiome (TM) has been linked to pancreatic cancer prognosis. Specific microbes can confer tumor resistance to therapies. Early knowledge of the TM at time of diagnosis would be clinically relevant for precision therapy based on microbial composition. However, it is difficult to define the TM prior to surgical resection. In this pilot feasibility study, patients underwent Endoscopic Ultrasound-Fine Needle Aspiration (EUS-FNA) biopsy of pancreatic adenocarcinoma. These samples were analyzed using 16S rRNA and internal transcribed spacer (ITS) sequencing for characterization of the tumor bacteria and fungi. After in silico decontamination and comparison to non-matched tumor, we were able to characterize the TM in biopsies, which was comparable to the TM from surgical specimens. EUS-FNA biopsy may represent a feasible modality to characterize the pancreatic TM prior to surgical resection with proper decontamination strategies and improvements in matched controls.
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