Oxidative ability and toxicity of n-hexane insoluble fraction of diesel exhaust particles.
Oxidative ability and toxicity of n-hexane insoluble fraction of diesel exhaust particles.
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柴油机尾气颗粒正己烷不溶部分的氧化能力和毒性。
DOI：
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发表时间：
2006
影响因子：
3.8
通讯作者：
Takahiro Kobayashi
中科院分区：
文献类型：
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作者：
Hirotoshi Shima;E. Koike;R. Shinohara;Takahiro Kobayashi
已知柴油机排气颗粒（DEP）会引起不良生物反应，例如气道炎症。然而，吸附在DEP上的有机化合物的化学特性与其生物效应之间的关系尚未完全了解。将DEP的二氯甲烷可溶部分（DMSF）分级为正己烷可溶部分（n-HSF）和正己烷不溶部分（n-HISF）。利用这些DEP组分，我们设计了本研究，以阐明（1）化学特性，（2）生物学特性，（3）化学和生物学特性之间的关系，这些DEP组分。采用二硫苏糖醇（DTT）、傅立叶变换红外光谱（FT-IR）、核磁共振氢谱（1H-NMR）和气相色谱-质谱（GC-MS）等方法对它们的化学性质进行了表征。血红素氧合酶-1（HO-1）蛋白表达，大鼠肺泡II型上皮细胞系（SV 40 T2）的活力，以及炎症细胞浸润到BALB/c小鼠腹腔分别作为氧化应激，细胞毒性和炎症反应的标志物进行了评价。DEP组分的氧化能力为n-HISF > DMSF > n-HSF。IR、1H-NMR和GC-MS分析表明，n-HISF主要由羟基和羰基等与氧化反应有关的官能团组成。HO-1蛋白表达、细胞毒性和炎症反应的相对强度也是n-HISF > DMSF > n-HSF。用N-乙酰-L-半胱氨酸（NAC）还原n-HISF诱导的所有生物活性均降低。这些结果表明，n-HISF具有高氧化能力和许多与氧合相关的官能团，并且这种能力强烈地有助于诱导氧化应激、细胞毒性和炎症反应。
Diesel exhaust particles (DEP) are known to induce adverse biological responses such as inflammation of the airway. However, the relationship between the chemical characteristics of organic compounds adsorbed on DEP and their biological effects is not yet fully understood. In this study, the dichloromethane-soluble fraction (DMSF) from DEP was fractionated into its n-hexane-soluble fraction (n-HSF) and n-hexane-insoluble fraction (n-HISF). Using these DEP fractions, we designed the present studies to elucidate (1) chemical characteristics, (2) biological characteristics, and (3) the relationship between the chemical and the biological characteristics of these DEP fractions. Dithiothreitol (DTT) assay, Fourier transform-infrared (FT-IR) spectroscopy, proton nuclear magnetic resonance (1H-NMR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS) were used to characterize their chemical properties. Heme oxygenase-1 (HO-1) protein expression, viability of rat alveolar type II epithelial cell line (SV40T2), and inflammatory cell infiltration into the peritoneal cavity of BALB/c mice were evaluated as markers of oxidative stress, cytotoxicity, and inflammatory response, respectively. The oxidative ability of the DEP fractions was n-HISF > DMSF > n-HSF. IR, 1H-NMR, and GC-MS spectra showed that n-HISF was mainly composed of compounds having many functional groups related to oxygenation, such as hydroxyl and carbonyl groups. The relative strength of HO-1 protein expression, cytotoxicity, and inflammatory responses was also n-HISF > DMSF > n-HSF. All of the n-HISF-induced biological activities were decreased by reduction with N-acetyl-L-cysteine (NAC). These results suggest that n-HISF has high oxidative ability and many functional groups related to oxygenation and that this ability strongly contributes to the induction of oxidative stress, cytotoxicity, and inflammatory response.
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影响因子：
120.7
作者：
Pope, CA;Burnett, RT;Thurston, GD
通讯作者：
Thurston, GD
DOI：
10.1164/ajrccm/151.3_pt_1.669
发表时间：
1995-03-01
影响因子：
24.7
作者：
POPE, CA;THUN, MJ;HEATH, CW
通讯作者：
HEATH, CW
活性氧和氮种在气道上皮对颗粒的反应中的作用。
影响因子：
10.4
作者：
通讯作者：
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影响因子：
158.5
作者：
DOCKERY, DW;POPE, CA;SPEIZER, FE
通讯作者：
SPEIZER, FE