Frequent multiplication of the long arm of chromosome 8 in hepatocellular carcinoma.-猫眼课题宝

肝细胞癌中 8 号染色体长臂的频繁增殖。

DOI：

发表时间：

1993

期刊：

Cancer Research

影响因子：

11.2

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

Y. Fujiwara;M. Monden;T. Mori;Y. Nakamura;M. Emi

在人类肝细胞癌中，已经检测到几条染色体上基因座的频繁等位基因丢失，但其他类型的染色体异常尚未得到很好的表征。我们使用8号染色体上的8个多态性DNA标记，检测了120例原发性肝细胞癌的肿瘤细胞中这些位点的拷贝数异常。在78个肿瘤中的32个中观察到代表单个等位基因的条带的强度增加2至6倍，所述条带为一个或多个标志物提供信息，表明8q上等位基因的拷贝数增加（“倍增”）。增殖的区域从几乎整个长臂到8号染色体的远端片段不等，但在所有32例病例中，8q24.11远端的末端区域都增殖。在56例结直肠癌中的9例中检测到8q上的类似倍增，这些肿瘤为一种或多种标记物提供信息。我们的研究表明，多态性DNA标记可用于检测细胞遗传学分析技术上困难的实体瘤中染色体材料的数值异常。

Frequent allelic losses at loci on several chromosomes have been detected in human hepatocellular carcinomas, but other types of chromosomal abnormalities have not been characterized well. Using eight polymorphic DNA markers on chromosome 8, we examined 120 primary hepatocellular carcinomas for abnormalities in the copy number of these loci in tumor cells. A 2- to 6-fold increase in intensities of bands representing single alleles was observed in 32 of the 78 tumors that were informative for one or more of the markers, indicating an increase in copy number ("multiplication") of alleles on 8q. The regions that multiplied varied from almost the entire long arm to a distal segment of chromosome 8, but a terminal region distal to 8q24.11 was multiplied in all 32 cases. Similar multiplications on 8q were detected in nine of 56 colorectal carcinomas that were informative for one or more of the markers. Our study demonstrated that polymorphic DNA markers can be used to detect numerical abnormalities of chromosomal material in solid tumors in which cytogenetic analysis is technically difficult.