High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis.
High expression of GOLPH3 in esophageal squamous cell carcinoma correlates with poor prognosis.
复制标题
GOLPH3 在食管鳞状细胞癌中的高表达与不良预后相关
DOI：
10.1371/journal.pone.0045622
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Song LB
中科院分区：
文献类型：
--
作者：
Wang JH;Chen XT;Wen ZS;Zheng M;Deng JM;Wang MZ;Lin HX;Chen K;Li J;Yun JP;Luo RZ;Song LB
高尔基体磷蛋白3（GOLPH 3）的表达是否与食管癌的发生有关目前尚不清楚。本研究旨在检测GOLPH 3在食管鳞状细胞癌（ESCC）中的表达，并探讨其临床意义。方法采用定量逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法（Western blotting）检测GOLPH 3在mRNA和蛋白质水平的表达差异。通过免疫组织化学方法测定食管鳞癌组织中GOLPH 3的表达水平，并根据患者和组织标本的具体临床病理特征进行比较。还分析了与患者生存相关的因素。结果GOLPH 3在食管鳞癌细胞系和食管鳞癌组织中的mRNA和蛋白表达水平均显著增高。GOLPH 3在食管鳞癌中的高表达与临床分期、TNM分期、组织分化程度及生存状态呈正相关（P均<0.0001）。GOLPH 3的表达被认为是ESCC患者的独立预后因素。表达高水平GOLPH 3的ESCC患者的5年总生存率显著低于GOLPH 3阴性患者。此外，GOLPH 3高表达和较短的总生存时间之间的显着相关性被发现在不同亚组的ESCC患者的临床分期，T分类，淋巴结转移分层。结论GOLPH 3可能参与ESCC的发生、分化和肿瘤发生，并可能作为ESCC的诊断和预后指标。
Background Whether the expression of Golgi phosphoprotein 3 (GOLPH3) correlates with esophageal cancer tumorigenesis is currently unclear. The aim of this study was to examine GOLPH3 expression in patients with esophageal squamous cell cancer (ESCC) and explore its clinical significance. Methods Differences in the expression of GOLPH3 at the mRNA and protein level were examined via quantitative reverse transcriptase PCR and western blotting, respectively. GOLPH3 expression levels in ESCC tissue were determined through immunohistochemistry, and were compared in accordance with specific clinicopathological features of the patients and tissue specimens. Factors associated with patient survival were also analyzed. Results A notably higher level of GOLPH3 expression was found in ESCC cell lines and tissues at both mRNA and protein levels. High expression of GOLPH3 in ESCC patients was positively associated with clinical stage, TNM classification, histological differentiation and vital status (all P<0.0001). Expression of GOLPH3 was found to be an independent prognostic factor in ESCC patients. ESCC patients expressing high levels of GOLPH3 exhibited a substantially lower 5-year overall survival than GOLPH3-negative patients. Furthermore, a significant correlation between high GOLPH3 expression and shorter overall survival time was found in different subgroups of ESCC patients stratified by the clinical stage, T classification, and lymph node metastasis. Conclusions Experiments demonstrated potential involvement of GOLPH3 in the development, differentiation, and tumorigenesis of ESCC, and concludes the possibility of its use as a diagnostic and prognostic marker in patients with ESCC.
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影响因子：
2.6
作者：
Tong, L.;Yuan, S.;Zhang, H.
通讯作者：
Zhang, H.
DOI：
10.1016/s1383-5742(00)00019-3
发表时间：
2000-04-01
影响因子：
5.3
作者：
Mandard, AM;Hainaut, P;Hollstein, M
通讯作者：
Hollstein, M
影响因子：
64.8
作者：
Scott, Kenneth L.;Kabbarah, Omar;Liang, Mei-Chih;Ivanova, Elena;Anagnostou, Valsamo;Wu, Joyce;Dhakal, Sabin;Wu, Min;Chen, Shujuan;Feinberg, Tamar;Huang, Joseph;Saci, Abdel;Widlund, Hans R.;Fisher, David E.;Xiao, Yonghong;Rimm, David L.;Protopopov, Alexei;Wong, Kwok-Kin;Chin, Lynda
通讯作者：
Chin, Lynda
DOI：
10.1158/1078-0432.ccr-09-1695
发表时间：
2010-04-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Scott KL;Chin L
通讯作者：
Chin L
影响因子：
11.2
作者：
Kim, Seok-Hyun;Nakagawa, Hiroshi;El-Deiry, Wafik S.
通讯作者：
El-Deiry, Wafik S.