RpS3 Is Required for Spermatogenesis of Drosophila melanogaster.
RpS3 Is Required for Spermatogenesis of Drosophila melanogaster.
复制标题
RPS3是果蝇的精子发生所必需的。
作者：
核糖体蛋白是核糖体的组成部分，参与蛋白质的生物合成过程。新出现的研究表明，许多RP在不同组织中表现出不同的表达水平，并在动物发育过程中以一种上下文相关的方式发挥作用。果蝇RPS3编码核糖体蛋白S3，它是核糖体40S亚基的一个组成部分。我们发现RPS3在成年果蝇的生殖器官中高表达，其在雄性生殖细胞中的缺失导致严重的精子产生和雄性生育能力的缺陷。免疫荧光染色显示，RPS3基因敲除对早期生殖细胞分化影响不大，但强烈干扰精子细胞的伸长和个体化过程。此外，我们观察到在RPS3基因敲除的睾丸中延长的精子细胞中线粒体衍生物的形态和活性异常，这可能导致轴突延长的失败。我们还发现，RPS3 RNAi抑制了负责解离精子细胞束的个性化复合体的形成。此外，在RPS3基因敲除的睾丸中检测到过多的凋亡细胞，这可能是为了清除有缺陷的精子细胞。综上所述，我们的数据表明，RPS3在调节精子细胞的伸长和个体化过程中发挥着重要作用，因此是正常果蝇精子发生所必需的。
Ribosomal proteins (RPs) constitute the ribosome, thus participating in the protein biosynthesis process. Emerging studies have suggested that many RPs exhibit different expression levels across various tissues and function in a context-dependent manner for animal development. Drosophila melanogaster RpS3 encodes the ribosomal protein S3, one component of the 40S subunit of ribosomes. We found that RpS3 is highly expressed in the reproductive organs of adult flies and its depletion in male germline cells led to severe defects in sperm production and male fertility. Immunofluorescence staining showed that RpS3 knockdown had little effect on early germ cell differentiation, but strongly disrupted the spermatid elongation and individualization processes. Furthermore, we observed abnormal morphology and activity of mitochondrial derivatives in the elongating spermatids of RpS3-knockdown testes, which could cause the failure of axoneme elongation. We also found that RpS3 RNAi inhibited the formation of the individualization complex that takes charge of disassociating the spermatid bundle. In addition, excessive apoptotic cells were detected in the RpS3-knockdown testes, possibly to clean the defective spermatids. Together, our data demonstrated that RpS3 plays an important role in regulating spermatid elongation and individualization processes and, therefore, is required for normal Drosophila spermatogenesis.
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影响因子：
11.8
作者：
Martin, Elliot T.;Blatt, Patrick;Nguyen, Elaine;Lahr, Roni;Selvam, Sangeetha;Yoon, Hyun Ah M.;Pocchiari, Tyler;Emtenani, Shamsi;Siekhaus, Daria E.;Berman, Andrea;Fuchs, Gabriele;Rangan, Prashanth
通讯作者：
Rangan, Prashanth
影响因子：
14.9
作者：
Hopes T;Norris K;Agapiou M;McCarthy CGP;Lewis PA;O'Connell MJ;Fontana J;Aspden JL
通讯作者：
Aspden JL
基于 F-肌动蛋白的头部囊细胞延伸物粘附在成熟的精子细胞上，使它们保持紧密的束状，并防止它们在果蝇睾丸中过早释放。
影响因子：
5.4
作者：
Desai BS;Shirolikar S;Ray K
通讯作者：
Ray K
影响因子：
2.9
作者：
Kibanov, Mikhail V.;Kotov, Alexei A.;Olenina, Ludmila V.
通讯作者：
Olenina, Ludmila V.
影响因子：
9.2
作者：
Noguchi, Tatsuhiko;Koizumi, Michiko;Hayashi, Shigeo
通讯作者：
Hayashi, Shigeo