F-actin-based extensions of the head cyst cell adhere to the maturing spermatids to maintain them in a tight bundle and prevent their premature release in Drosophila testis.
F-actin-based extensions of the head cyst cell adhere to the maturing spermatids to maintain them in a tight bundle and prevent their premature release in Drosophila testis.
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基于 F-肌动蛋白的头部囊细胞延伸物粘附在成熟的精子细胞上，使它们保持紧密的束状，并防止它们在果蝇睾丸中过早释放。
DOI：
10.1186/1741-7007-7-19
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发表时间：
2009-05-05
期刊：
影响因子：
5.4
通讯作者：
Ray K
中科院分区：
文献类型：
--
作者：
Desai BS;Shirolikar S;Ray K
在果蝇中，所有64个克隆来源的精母细胞在两个体源性囊肿细胞内的合胞体中分化。它们拉长形成细长的精细胞，这些精细胞被个体化，然后释放到精囊中。在个体化过程中，分化的精子在囊肿内被组织成一个紧密的束，这在精子选择中发挥了重要作用。然而，这一过程的实际意义及其潜在机制尚不清楚。我们发现动态的基于f -肌动蛋白的过程在个体化开始时从头囊肿细胞延伸，填充成熟精子束吻端间隙。除肌动蛋白外，这些结构还含有层粘连蛋白、-连环蛋白、动力蛋白、肌凝蛋白VI和其他一些丝状结构成分。此外，药理学和遗传学分析表明，细胞骨架稳定性和动力蛋白功能对其维持至关重要。这些基于f -肌动蛋白的过程的破坏与精子束解体和睾丸内精子过早释放有关。总之，我们的数据表明，头部囊肿细胞通过基于f -肌动蛋白的延伸附着在成熟的精子头上，从而使它们保持在一个紧密的束中。这可能会调节成熟精子释放到精囊中。总的来说，这一过程与哺乳动物的精子受精相似。
In Drosophila, all the 64 clonally derived spermatocytes differentiate in syncytium inside two somatic-origin cyst cells. They elongate to form slender spermatids, which are individualized and then released into the seminal vesicle. During individualization, differentiating spermatids are organized in a tight bundle inside the cyst, which is expected to play an important role in sperm selection. However, actual significance of this process and its underlying mechanism are unclear. We show that dynamic F-actin-based processes extend from the head cyst cell at the start of individualization, filling the interstitial space at the rostral ends of the maturing spermatid bundle. In addition to actin, these structures contained lamin, beta-catenin, dynamin, myosin VI and several other filopodial components. Further, pharmacological and genetic analyses showed that cytoskeletal stability and dynamin function are essential for their maintenance. Disruption of these F-actin based processes was associated with spermatid bundle disassembly and premature sperm release inside the testis. Altogether, our data suggests that the head cyst cell adheres to the maturing spermatid heads through F-actin-based extensions, thus maintaining them in a tight bundle. This is likely to regulate mature sperm release into the seminal vesicle. Overall, this process bears resemblance to mammalian spermiation.
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影响因子：
11.8
作者：
Arama, E;Agapite, J;Steller, H
通讯作者：
Steller, H
影响因子：
8
作者：
Gvakharia, BO;Bebas, P;Giebultowicz, JM
通讯作者：
Giebultowicz, JM
影响因子：
3.6
作者：
Guttman, JA;Takai, Y;Vogl, AW
通讯作者：
Vogl, AW
DOI：
10.1073/pnas.0602443103
发表时间：
2006-08-15
影响因子：
11.1
作者：
Bohil, Aparna B.;Robertson, Brian W.;Cheney, Richard E.
通讯作者：
Cheney, Richard E.
影响因子：
7.5
作者：
Faix, J;Rottner, K
通讯作者：
Rottner, K