A Simple and Rapid Low-Cost Procedure for Detection of Vancomycin-Resistance Genes in Enterococci Reveals an Outbreak of Vancomycin-Variable Enterococcus faecium.
A Simple and Rapid Low-Cost Procedure for Detection of Vancomycin-Resistance Genes in Enterococci Reveals an Outbreak of Vancomycin-Variable Enterococcus faecium.
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DOI：
10.3390/diagnostics12092120
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发表时间：
2022-08-31
期刊：
影响因子：
--
通讯作者：
Kemp M
中科院分区：
文献类型：
--
作者：
Abdullah HM;Marbjerg LH;Andersen L;Hoegh SV;Kemp M
检测从患者身上培养的肠球菌对万古霉素的耐药性对个体患者的治疗和预防医院传播具有重要意义。表型抗菌素耐药试验可能无法检测出潜在的万古霉素耐药肠球菌。我们已经开发并测试了一种基于PCR的程序，用于在肠球菌临床样本中常规筛查万古霉素耐药基因。在未分离特异性细菌的情况下，用实时荧光定量PCR技术检测诊断标本中存在粪肠球菌或脸孢杆菌的原代培养物的vanA和vanB基因。在每个实时PCR反应中，最多收集10个样本并进行测试，随后对阳性池中的培养物进行单独测试。在2017年为期一个月的测试期间，在340份诊断培养报告的万古霉素敏感肠球菌尿样中，有一份尿样中检测到vanA基因。2018年的第二个测试期包括357份尿液样本，在8名患者的样本中检测到vanA基因。随后，对所有报告在一年内有粪肠杆菌生长的尿液样本进行了检测。58人被鉴定出携带以前未检测到的vanA基因的肠球菌。对患者标本原代培养物进行常规分子检测可提高对携带万古霉素耐药基因的住院患者粪肠杆菌的检出率。
The detection of resistance to vancomycin in enterococci cultured from patients is important for the treatment of individual patients and for the prevention of hospital transmission. Phenotypic antimicrobial resistance tests may fail to detect potential vancomycin-resistant enterococci. We have developed and tested a PCR based procedure for routine screening for vancomycin-resistance genes in clinical samples with enterococci. Primary cultures from diagnostic samples reported with growth of Enterococcus faecium or E. facalis were tested for vanA and vanB genes by real-time PCR without the isolation of specific bacteria. Up to ten samples were pooled and tested in each real-time PCR reaction, with subsequent individual testing of cultures from positive pools. In a one-month test period in 2017 vanA gene was detected in one out of 340 urine samples with vancomycin-susceptible enterococci reported from diagnostic culture. A second test period in 2018 included 357 urine samples, and vanA gene was detected in samples from eight patients. Subsequently, all urine samples reported with growth of E. faecium during a period of one year were tested. Fifty-eight individuals were identified with enterococci, carrying the vanA gene not previously detected. Routine molecular testing of primary culture material from patient samples may improve the detection of hospitalized patients carrying E. faecium with resistance genes to vancomycin.
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影响因子：
4.9
作者：
Graham, M.;Ballard, S. A.;Grayson, M. L.
通讯作者：
Grayson, M. L.
影响因子：
9.4
作者：
Hegstad, Kristin;Giske, Christian G.;Sundsfjord, Arnfinn
通讯作者：
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影响因子：
5.2
作者：
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通讯作者：
Roger, Michel
DOI：
10.1007/s10096-011-1304-0
发表时间：
2012-03-01
影响因子：
4.5
作者：
Fang, H.;Ohlsson, A. -K.;Ullberg, M.
通讯作者：
Ullberg, M.
影响因子：
9.4
作者：
Mak, Anton;Miller, Mark A.;Monczak, Yury
通讯作者：
Monczak, Yury