Traffic of p24 Proteins and COPII Coat Composition Mutually Influence Membrane Scaffolding.
Traffic of p24 Proteins and COPII Coat Composition Mutually Influence Membrane Scaffolding.
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DOI：
10.1016/j.cub.2015.03.029
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发表时间：
2015-05-18
期刊：
影响因子：
9.2
通讯作者：
Miller, Elizabeth A.
中科院分区：
文献类型：
--
作者：
D'Arcangelo, Jennifer G.;Crissman, Jonathan;Pagant, Silvere;Copic, Alenka;Latham, Catherine F.;Snapp, Erik L.;Miller, Elizabeth A.
真核生物的蛋白质分泌需要高效、准确地输送不同的分泌和膜蛋白。这一过程始于内质网，囊泡由必需的COPII外衣雕刻而成。COPII膜的Sec13p亚基有助于膜支架的形成，使新生囊泡发生弯曲。当腔定向膜蛋白的运输被取消时，对Sec13p的需求可以被绕过。在这里，我们试图进一步探索货运蛋白对囊泡形成的影响。我们表明，p24家族蛋白质的有效内质网输出是对Sec13p需求的主要驱动因素。P24复合体提供的支架负担部分由货物适配器Lst1p满足，该适配器与包括p24蛋白在内的货物子集结合。我们认为，Lst1p的支架功能是产生囊泡所必需的，这些囊泡可以容纳困难的货物蛋白，包括大的寡聚组装和不对称分布的膜蛋白。含有此类货物的囊泡也更依赖于Sec13P的支架，并可作为其他系统中大型载体形成的模型。
Eukaryotic protein secretion requires efficient and accurate delivery of diverse secretory and membrane proteins. This process initiates in the endoplasmic reticulum, where vesicles are sculpted by the essential COPII coat. The Sec13p subunit of the COPII coat contributes to membrane scaffolding, which enforces curvature on the nascent vesicle. A requirement for Sec13p can be bypassed when traffic of lumenally oriented membrane proteins is abrogated. Here, we sought to further explore the impact of cargo proteins on vesicle formation. We show that efficient ER export of the p24 family of proteins is a major driver of the requirement for Sec13p. The scaffolding burden presented by the p24 complex is met in part by the cargo adaptor, Lst1p, which binds to a subset of cargo, including the p24 proteins. We propose that the scaffolding function of Lst1p is required to generate vesicles that can accommodate difficult cargo proteins that include large oligomeric assemblies and asymmetrically distributed membrane proteins. Vesicles that contain such cargoes are also more dependent on scaffolding by Sec13p and may serve as a model for large carrier formation in other systems.
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影响因子：
3.3
作者：
Copic, Alenka;Dorrington, Mariana;Miller, Elizabeth A.
通讯作者：
Miller, Elizabeth A.
影响因子：
46.9
作者：
Pédelacq, JD;Cabantous, S;Waldo, GS
通讯作者：
Waldo, GS
影响因子：
64.5
作者：
Muñiz, M;Morsomme, P;Riezman, H
通讯作者：
Riezman, H
DOI：
10.1126/science.1215909
发表时间：
2012-03-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Copic A;Latham CF;Horlbeck MA;D'Arcangelo JG;Miller EA
通讯作者：
Miller EA
影响因子：
4.8
作者：
Belden, WJ;Barlowe, C
通讯作者：
Barlowe, C