Biochar and microbial signaling: production conditions determine effects on microbial communication.
Biochar and microbial signaling: production conditions determine effects on microbial communication.
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DOI：
10.1021/es401458s
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发表时间：
2013-10-15
影响因子：
11.4
通讯作者：
Silberg, Jonathan J.
中科院分区：
文献类型：
--
作者：
Masiello, Caroline A.;Chen, Ye;Gao, Xiaodong;Liu, Shirley;Cheng, Hsiao-Ying;Bennett, Matthew R.;Rudgers, Jennifer A.;Wagner, Daniel S.;Zygourakis, Kyriacos;Silberg, Jonathan J.
木炭具有较长的土壤停留时间，这导致了它作为碳固存技术（生物炭）的生产和使用。土壤生物炭可以引发一系列生物效应，对碳储量产生积极和消极的影响，例如改变有机物的分解速度和改变植物生物量产量。细胞信号的吸收已经被假设为这些效应的基础，但生物化学信号的结合是否发生仍然未知，如果是这样，在与微生物生长和交流相关的时间尺度上。我们研究了生物炭对n -3-氧十二烷基- l-高丝氨酸内酯（AHL）细胞间信号分子的吸附，这种分子被许多革兰氏阴性土壤微生物用来调节基因表达。我们表明，木材生物炭破坏生长中的多细胞系统内的通信，该系统由合成AHL的发送细胞和响应AHL信号表达绿色荧光蛋白的接收细胞组成。然而，生物炭对ahl介导的细胞间通讯的抑制作用各不相同，在700°C（表面积为301 m2/g）下制备的生物炭对细胞通讯的抑制作用是在300°C（表面积为3 m2/g）下制备的同等质量的生物炭的10倍。这些发现提供了第一个直接证据，表明生物炭对依赖于细胞间信号的基因表达产生了一系列影响，这意味着生物炭制备方法可以作为一个参数来调节微生物依赖的土壤过程，如固氮和根茎作物的害虫攻击。
Charcoal has a long soil residence time, which has resulted in its production and use as a carbon sequestration technique (biochar). A range of biological effects can be triggered by soil biochar that can positively and negatively influence carbon storage, such as changing the decomposition rate of organic matter and altering plant biomass production. Sorption of cellular signals has been hypothesized to underlie some of these effects, but it remains unknown whether the binding of biochemical signals occurs, and if so, on time scales relevant to microbial growth and communication. We examined biochar sorption of N-3-oxo-dodecanoyl-L-homoserine lactone, an acyl-homoserine lactone (AHL) intercellular signaling molecule used by many gram-negative soil microbes to regulate gene expression. We show that wood biochars disrupt communication within a growing multicellular system that is made up of sender cells that synthesize AHL and receiver cells that express green fluorescent protein in response to an AHL signal. However, biochar inhibition of AHL-mediated cell-cell communication varied, with the biochar prepared at 700°C (surface area of 301 m2/g) inhibiting cellular communication 10-fold more than an equivalent mass of biochar prepared at 300°C (surface area of 3 m2/g). These findings provide the first direct evidence that biochars elicit a range of effects on gene expression dependent on intercellular signaling, implicating the method of biochar preparation as a parameter that could be tuned to regulate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops.
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影响因子：
3.2
作者：
Elad, Yigal;David, Dalia Rav;Graber, Ellen R.
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