Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility.
Drought and Heat Differentially Affect XTH Expression and XET Activity and Action in 3-Day-Old Seedlings of Durum Wheat Cultivars with Different Stress Susceptibility.
复制标题
DOI：
10.3389/fpls.2016.01686
复制
发表时间：
2016
影响因子：
5.6
通讯作者：
Lenucci MS
中科院分区：
文献类型：
--
作者：
Iurlaro A;De Caroli M;Sabella E;De Pascali M;Rampino P;De Bellis L;Perrotta C;Dalessandro G;Piro G;Fry SC;Lenucci MS
高温和干旱胁迫已成为硬粒小麦生产的主要限制因素。在地中海地区，它们对作物生产力的负面影响预计将因正在发生的气候变化而加剧。木葡聚糖内切转葡糖基酶/水解酶（XTH）是细胞壁重塑的主要酶，其在细胞扩张和形态发生中的相关性表明在应激反应中起核心作用。在这项工作中，XTH在非生物胁迫耐受性的硬质小麦的潜在作用进行了研究。脱水和热暴露对XTH表达及其内转葡萄糖基酶（XET）的体外活性和体内作用的单独影响已被监测，长达24小时，在顶端和亚顶端根区和芽切离3天的硬粒小麦品种不同的胁迫敏感性/耐受性的幼苗。脱水和热胁迫差异影响XTH的表达谱和XET的活性和行动在小麦幼苗，取决于敏感性/耐受性的品种，器官，根的拓扑区域的程度，在根内，细胞分化的梯度。根尖区域是所有测定品种中主要受两种处理影响的区域，而在地上部水平观察到XET活性没有变化，无论敏感性/耐受性如何，证实了根在胁迫感知、信号传导和响应中的关键作用。根据应力类型观察到脱水效应：脱水引起的整体增加，至少在根尖区域的根，XET活性和行动，而在大多数品种的热处理引起显着的抑制。这些数据表明，XET行动中定义的部分年轻的硬粒小麦幼苗的根的差异变化可能有一个作用，作为对干旱和热胁迫的响应，从而有助于幼苗存活和作物建立。深入了解这些变化的机制可能是实施育种策略以开发适应未来气候情景的新的高产杂交种的理论基础。
Heat and drought stress have emerged as major constraints for durum wheat production. In the Mediterranean area, their negative effect on crop productivity is expected to be exacerbated by the occurring climate change. Xyloglucan endotransglucosylase/hydrolases (XTHs) are chief enzymes in cell wall remodeling, whose relevance in cell expansion and morphogenesis suggests a central role in stress responses. In this work the potential role of XTHs in abiotic stress tolerance was investigated in durum wheat. The separate effects of dehydration and heat exposure on XTH expression and its endotransglucosylase (XET) in vitro activity and in vivo action have been monitored, up to 24 h, in the apical and sub-apical root regions and shoots excised from 3-day-old seedlings of durum wheat cultivars differing in stress susceptibility/tolerance. Dehydration and heat stress differentially influence the XTH expression profiles and the activity and action of XET in the wheat seedlings, depending on the degree of susceptibility/tolerance of the cultivars, the organ, the topological region of the root and, within the root, on the gradient of cell differentiation. The root apical region was the zone mainly affected by both treatments in all assayed cultivars, while no change in XET activity was observed at shoot level, irrespective of susceptibility/tolerance, confirming the pivotal role of the root in stress perception, signaling, and response. Conflicting effects were observed depending on stress type: dehydration evoked an overall increase, at least in the apical region of the root, of XET activity and action, while a significant inhibition was caused by heat treatment in most cultivars. The data suggest that differential changes in XET action in defined portions of the root of young durum wheat seedlings may have a role as a response to drought and heat stress, thus contributing to seedling survival and crop establishment. A thorough understanding of the mechanisms underlying these variations could represent the theoretical basis for implementing breeding strategies to develop new highly productive hybrids adapted to future climate scenarios.
登录
查看更多内容
影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
影响因子：
--
作者：
De Caroli, Monica;Lenucci, Marcello S.;Piro, Gabriella
通讯作者：
Piro, Gabriella
影响因子：
4.2
作者：
Hara, Yoshinao;Yokoyama, Ryusuke;Nishitani, Kazuhiko
通讯作者：
Nishitani, Kazuhiko
影响因子：
7.4
作者：
Antosiewicz, DM;Purugganan, MM;Braam, J
通讯作者：
Braam, J
影响因子：
3.8
作者：
Frankova, Lenka;Fry, Stephen C.
通讯作者：
Fry, Stephen C.